| 研究課題/領域番号 |
18F18386
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
佐瀬 英俊 沖縄科学技術大学院大学, 植物エピジェネティクスユニット, 准教授 (70510006)
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| 研究分担者 |
MIRYEGANEH NODE MATIN 沖縄科学技術大学院大学, 植物エピジェネティクスユニット, 外国人特別研究員
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| 研究期間 (年度) |
2018-11-09 – 2021-03-31
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| キーワード | Epigenetics / Plants / Environment |
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| 研究実績の概要 |
We are planning to investigate how methylation variation is distributed among individuals from different habitats in different level of stress, and how this variation is correlated with their morphological differences and gene expression.
In order to do this we first sequenced the genomes and transcriptomes of representative mangrove species using the combination of long read sequencing technology (pacbio) supplemented by the more conventional platforms (Illumina Hiseq and matepair) to correct for the error of long read sequencing. To produce a high-quality reference genome, we performed a de novo assembly using these sequencing data.
We have also started a de novo RNA seq analysis and complementary detailed de novo annotation of genes based on RNA sequencing data.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The genome assembly and annotation process has been completed as planned. We also completed gene expression analysis to identify genomic characteristics that are conserved within each group of plants but differ among them and now trying to reveal differences in the transcriptome profiles among populations that are growing under different level of stress.
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| 今後の研究の推進方策 |
We will try to integrate epigenome data, and understand impact of environmental changes on epigenome dynamics in mangrove genome.
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