| 研究課題/領域番号 |
18F18391
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| 研究機関 | 岡山大学 |
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研究代表者 |
森 泉 岡山大学, 資源植物科学研究所, 准教授 (40379805)
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| 研究分担者 |
OOI LIA 岡山大学, 資源植物科学研究所, 外国人特別研究員
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| 研究期間 (年度) |
2018-11-09 – 2021-03-31
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| キーワード | sulfur oxide / stomata |
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| 研究実績の概要 |
Plants have a mechanism to cope with the invasion of toxic gasses through stomatal opening. It is known that stomata close against sulfur oxide as well as ozone. The molecular mechanisms for ozone-inudced stomatal closure. However, sulfur dioxide-induced stomatal closure mechanism has not been understood. In this study we analyzed the response of stomata to sulfur dioxide in the mutants of which stomata is insensitive to ozone to compare the molecular mechanism of ozone- and sulfur dioxide-induced stomatal closure. We found that ozone and carbon dioxide-induced stomatal closure mechanisms are mostly shred, while sulfur dioxide-induced stomatal closure mechanism is different.
Since some plant hormones have been postulated to play a role in sulfur dioxide-induced stomatal closure, we analyzed hormone contents in sulfur dioxide-exposed leaf by liquid-chromatography mass spectrometry. We found that concentrations of jasmonic acid and indoleacetic acid showed significant change. Indoleacetic acid did not induced stomatal closure. While jasmonic acid induced closure, jasmonic acid-insensitive mutant plant showed an essentially same response to sulfur dioxide, suggesting that jasmonic acid is not involved in sulfur dioxide-induced stomatal closure.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
We analyzed the involvement of nitric oxide and reactive oxygen species in addition to plant hormones. Our result showed no significant involvement of nitric oxide and reactive oxygen species. We successively analyzed the involvement of cell acidification in this process. We found that mutants demonstrating strongly stable cytosolic pH are less sensitive to sulfur dioxide with respect to stomatal behavior. Based on our results, we propose a novel biotechnology for plants/crops to cope with sulfur dioxide (acid rain) stress.
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| 今後の研究の推進方策 |
Investigation of the involvement of CLCa gene in sulfur dioxide detoxification and enhancement of sulfur dioxide tolerance. Previous studies were conducted using excised Arabidopsis leaves floated on sulfur dioxide-producing buffer to examine the effects of sulfur dioxide on stomatal physiology. Next, sulfur dioxide impacts on whole plants will be investigated using gas chamber fumigation approach. Whole plants of wild-types and mutants will be incubated with sulfur dioxide gas in a gas chamber. The effects of sulfur dioxide on chlorophyll contents and cell viability will be investigated. This study is expected to provide insights on plant resistant mechanisms against sulfur dioxide and potential approach to improve sulfur dioxide resistance.
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