| 研究課題/領域番号 |
18F18721
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Carninci Piero 国立研究開発法人理化学研究所, 生命医科学研究センター, 副センター長 (10333296)
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| 研究分担者 |
ROBBE PAULINE 国立研究開発法人理化学研究所, 生命医科学研究センター, 外国人特別研究員
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| 研究期間 (年度) |
2018-07-25 – 2021-03-31
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| キーワード | Transcriptomics / Genomics / cancer / Non-coding RNA / Non-coding mutations |
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| 研究実績の概要 |
The project planned in the beginning of the fellowship on hepatocellular carcinoma has been continued. The focus was made in learning bioinformatics techniques in order to analyse the biological data.
By combining information from public databases and data from hepatocellular carcinoma primary samples, a list of non-coding regulatory elements, specifically named enhancers was prepared. Several of these enhancers were found with many DNA alterations, or mutations which are absent in the normal liver tissues, and more than expected by chance by applying multiple statistical approaches to uncover them. Next, the region of interest were investigated by transcriptomics techniques. Additional experimental validation are also being planned.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I have completed Aim 1: I have constructed a bioinformatics pipeline to analyze CAGE data and defined a robust and permissive maps of HCC specific enhancers. The results need discussion and revision.
I have partially completed Aim 2: I have finalized the list of non-coding regions potentially altered in HCC using whole genome sequencing. I have processed the RNA-seq data in order to validate some of the non-coding regions. I need to acquire additional skills in bioinformatics to finalise the work.
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| 今後の研究の推進方策 |
To finalise the study, co-expression analysis will be conducted in order to link enhancers and target genes. Finally, experimental validated of selected targets should be performed in the lab.
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