| 研究実績の概要 |
The three pathogenic bacterial strains have been purchased, delivered, successfully cultured and stored at Kagawa University, according to the relevant regulations. Genomic DNA has been successfully extracted from the strains. WzaCA is transporter of colonic acid. Polymerase chain reaction has allowed the WzaCA gene copied from the genomic DNA and subsequent cloning onto desired vectors. Expression of the WzaCA is currently in progress. On the other hand, various genomic mutants are being created with a scarless genome editing technique. Single-molecule setup for the Wza pores has been established. An invited book chapter related to this project is in press:
Lingbing Kong, Revelation of function and inhibition of Wza through single channel studies, Methods Mol. Biol., 2019, in press.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
There was delay in the delivery of some equipment, strains and reagents, which led to the delay of some experiments. However, most of the scheduled goals have been achieved. The implementation of the following scheduled research plans will therefore be fine.
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