| 研究課題/領域番号 |
18H02425
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Wu Yibo 国立研究開発法人理化学研究所, 生命医科学研究センター, 上級研究員 (50811618)
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| 研究期間 (年度) |
2018-04-01 – 2021-03-31
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| キーワード | proteomics / obesity / adipose immune cells / adipocytes |
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| 研究実績の概要 |
Adipose tissue plays an important role in sensing and managing energy homeostasis. Adipose tissue immune cells coordinate with adipocytes to keep the energy balance in lean status, while the dysregulation of adipose immune cells may result in adipose tissue inflammation and obesity. Scientists have made significant progress in identifying the infiltration, activation and functions of adipose immune cells during the development of obesity. However, the detailed roles of different immune cells in adipose tissue remain unclear.
Quantitative proteomics measurements by mass spectrometry, especially Data-Independent Acquisition Mass Spectrometry (DIA-MS), have shown unprecedented quantitation accuracy and proteome coverage in complex biological systems. In the current study, we established the diet-induced obesity (DIO) model mice. Using fluorescence-activated cell sorting (FACS), we sorted adipose tissue macrophages from visceral adipose tissue from the DIO and control mice and analyzed them separately using DIA-MS strategy. Our data revealed dramatic proteome difference between macrophages from obese and control mice and suggested novel protein markers that regulate macrophage activation in obesity.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have modified the research plan based on the proposed one. We analyzed some tissue samples from aging mice, but we found the age-related proteome changes were quite small. Thus, we decided to use mice models with more distinguishable proteome profiles. In the current experiment, we have established the diet-induced obesity (DIO) mice model using the B6N mice. We have set up the workflow from adipose immune cell isolation to protein and peptide sample preparation, as well as mass spectrometry analysis of each isolated cell type. We have also performed preliminary data analysis of the required proteomics data. We consider it as a reasonable progress for the first year of the project.
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| 今後の研究の推進方策 |
We have acquired the first batches of proteomics data from the current experiment. We plan to further optimize the workflow that is described above, and apply it to DIO mice at different diet treatment time points. Furthermore, as the preliminary proteomics data have suggested significant metabolic changes between macrophages from the DIO and control mice, we plan to perform metabolomics analysis for the same samples. We will also isolate more adipose immune cell types in addition to macrophages, and perform similar analysis using these cell types. We will integrate the results from different immune cell types and adipocytes to generate the interaction network between them in lean and obesity.
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