| 研究実績の概要 |
In FY2019, we have generated time-course cell type-specific proteomics data for adipose tissue cells comparing high-fat and control diet fed mice, and have found a few candidate genes. In FY2020, we focused on validating function of the chloride intracellular channel protein 4 (Clic4) in macrophages. Interestingly, in apoptotic adipocyte (apoAP)-treated RAW 264.7 cells, we observed increased lipid accumulation upon Clic4 knockdown. We then performed proteomic and lipidomic analyses and found enhanced OXPHOS in Clic4-deficient macrophages. Besides, we expanded the analysis to study ligand-receptor pairs for both intercellular communication and autocrine of the 5 analyzed cell types. We found ligand-receptor pairs that were significantly regulated by diet in a time-dependent manner.
|
