| 研究課題/領域番号 |
18H02452
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| 研究機関 | 熊本大学 |
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研究代表者 |
Sheng Guojun 熊本大学, 国際先端医学研究機構, 特別招聘教授 (20399439)
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| 研究期間 (年度) |
2018-04-01 – 2021-03-31
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| キーワード | mesothelium / chicken / chorioallantois / EMT |
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| 研究実績の概要 |
In the second year of this project (2019 fiscal year), we have achieved major parts of aims 2-4. More specifically, we have uncovered involvement of canonical EMT regulatory gene (SNAI1) and non-canonical EMT regulatory gene (WT1) during CAM fusion. We have performed gene expression analysis of those transcription factors. We have also made CRISPR mediated gene activation (CRISPR-a) and inhibition (CRISPR-i) constructs for these two genes and performed gain- and loss-of function studies in CAM culture ex ovo. We have optimized ex vivo CAM culture and DNA construct electroporation methodology. We have performed immunofluorescence studies of CAM samples for EMT marker gene expression and have performed electron microscopy studies of fusing CAM tissues for cell biological studies.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The project is making progress as we have originally planned.
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| 今後の研究の推進方策 |
The final year of this project, we will clarify the difference between allantoic mesothelial EMT and chorionic mesothelial EMT. We will complete the functional study of WT1 and SNAI1 in mesothelial EMT fusion. We will also complete the EM morphological study of CAM fusion. We will summarize our data and prepare manuscript for submission in the 2020 fiscal year.
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