| 研究実績の概要 |
Histamine H1 receptors (H1Rs) were shown to contribute to various physiological processes, however the role of astrocytic H1Rs in brain function remained unknown.
In order to investigate the role of astrocytic H1Rs, I successfully established and validated novel conditional knock out (cKO) mouse models that lack H1Rs selectively on astrocytes or neurons. There were no obvious differences of cKO mice (e.g. body weight, size). Genetic analysis confirmed H1R gene deficiency in targeted cells.
I carried out behavioural assessment and found significant changes in cKO mice: cKO mice showed significant changes in memory, aggressive behavior, quality of wakefulness, and anxiety. For the first time, physiological processes could be attributed to either neuronal or astrocytic H1R signalling.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The project is slightly delayed as establishing molecular imaging techniques that show both, high sensitivity and specificity did not work as expected. A positive outcome of these experiments, that included immunohistochemistry and in-situ hybridization, was desirable because it increases the significance of the study.
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