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2018 年度 実績報告書

植物・微生物間の相互作用の解明による土壌中のヒ素を効率的に除去する技術の確立

研究課題

研究課題/領域番号 18J11625
研究機関東北大学

研究代表者

YANG CHONGYANG  東北大学, 環境科学研究科, 特別研究員(DC2)

研究期間 (年度) 2018-04-25 – 2020-03-31
キーワードarsenic / rhizobacteria / Pteris vittata
研究実績の概要

The research went smoothly as planned. In this year, I focused on rhizosphere of arsenic hyperaccumulators Pteris vittata and Pteris multifida and role of rhizosphere bacteria to elucidate the interactions of plants and rhizobacteria. The aim of this study was to investigate the potential of rhizobacteria in enhancing the effect of arsenic phytoextraction. Seventy-eight strains rhizobacteria was isolated and identified by blasting to gene bank. Their traits were characterized to select the optimal isolate. The interactions of selected multifunctional strain with plants were exhibited positive effects. Further, co-cultivation of rhizobacterium and ferns achieved the promotion arsenic phytoremediation. I wrote two papers, one was published, another one was under reviewing. According to papers writing, I obtained a deeper understanding of research, which can lay a solid foundation for further research plan. And I attended an international conference and national conference. Academic communication provides the enlighten thoughts and ideas about my research.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

The title of my research is “Study on rhizosphere of arsenic hyperaccumulators forward to develop an efficient phytoremediation technology”. I mainly focused on rhizosphere of arsenic hyperaccumulators Pteris vittata and Pteris multifida and role of rhizosphere bacteria to elucidate the interactions of plants and rhizobacteria. The aim of this study was to investigate the potential of rhizobacteria in enhancing the effect of arsenic phytoextraction. And base on it, rhizobacteria was isolated and identified by blasting to gene bank. Their traits were characterized to select the optimal isolate. The interactions of selected multifunctional strain with plants were exhibited positive effects. Further, co-cultivation of rhizobacterium and ferns achieved the promotion arsenic phytoremediation. The research went smoothly as planned. Each experiment was conducted after a lot of relevant literatures investigating, independent thinking and communication with professor or laboratory members.

今後の研究の推進方策

(1)Molecular analysis will be applied in rhizosphere samples of co-cultivation field trail. A aioA operon which promotes As(III) to As(V) will be detected as target gene to calculate the abundance of bacteria with aio gene in rhizosphere after the inoculation.
(2) Pot experiment will be applied to control needless variable in laboratory. Next generation sequence will be analyzed to detect the microbial diversity and richness with functional strain inoculation and non-inoculation.
(3)Summarized 3 years of phytoremediation with functional strain inoculation. Soil arsenic will be detected to compare changeable of 3 years phytoremediation. Proposal of long-term phytoremediation will be offered and optimized for further study.

  • 研究成果

    (3件)

すべて 2018

すべて 雑誌論文 (1件) 学会発表 (2件) (うち国際学会 1件)

  • [雑誌論文] 根圏微生物が引き出すファイトレメディエーションのさらなる可能性2018

    • 著者名/発表者名
      楊重陽,簡梅芳
    • 雑誌名

      アグリバイオ

      巻: 2 ページ: 56-57

  • [学会発表] A multifunctional rhizobacterium, Pseudomonas vancouverensis strain DhA-51, increases arsenic extraction by Pteris vittata2018

    • 著者名/発表者名
      Chongyang Yang, Meifang Chien, Chihiro Inoue
    • 学会等名
      環境バイオテクノロジー学会
  • [学会発表] Efficient Arsenic Phytoremediation by Co-culture of Pteris Multifida with a Multifunctional Rhizobacterium2018

    • 著者名/発表者名
      Chongyang Yang, Meifang Chien, Chihiro Inoue
    • 学会等名
      15th International Phytotechnology Conference
    • 国際学会

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公開日: 2019-12-27  

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