膵再生医療に向けた立体膵組織の作製技術の確立

2018 年度 実績報告書

• 研究課題/領域番号: 18J11639
• 研究機関: 横浜国立大学
• 研究代表者: MYASNIKOVA Dina 横浜国立大学, 工学府, 特別研究員(DC2)
• 研究期間 (年度): 2018-04-25 – 2020-03-31
• キーワード: pancreas / insulin / ROS / antioxidant

研究実績の概要

It was confirmed that culturing pancreatic spheroids in a PDMS-chip leads to increase in ROS production compared with spheroids cultured in a plastic chip or in monolayer. Therefore, conditions for culturing pancreatic spheroids in PDMS-chip were optimised by adding antioxidant ascorbic acid-2-phosphate and comparing its action with two other antioxidants (n-acetylcysteine and dithiothreitol). The addition of antioxidants led to the increase in insulin production.
The possibility of formation of vascularised pancreatic spheroids in collagen gel was shown by embedding MIN6 and fibroblasts co-culture spheroids into collagen gel with dispersed pancreatic microvascular endothelial cells.

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

As it was planned, I finished the work with antioxidants. The results were published in a paper and presented at international conference.
I also started the work with vascularised spheroids and neuro-vascularture.

今後の研究の推進方策

First, I want to investigate the influence of glucose concentration on MIN6 spheroids during their culture, as pancreatic beta-cells are known to be sensitive to glucose concentration.
Another direction is to continue investigate interactions between pancreatic, vascular and neuron cells. As my research showed, it is difficult to use PC12 (neuron cell line) for this purpose. Thus, I will need to learn how to work with primary or stem cells.

研究成果

• [雑誌論文] Synergic effects of oxygen supply and antioxidants on pancreatic β-cell spheroids (2019)
  • 著者名/発表者名: Dina Myasnikova, Tatsuya Osaki, Kisaki Onishi, Tatsuto Kageyama, Binbin Zhang Molino, Junji Fukuda
  • 雑誌名: Scientific reports 巻: 9 ページ: 1802
  • DOI: https://doi.org/10.1038/s41598-018-38011-6
  • 査読あり / オープンアクセス / 国際共著
• [学会発表] Ascorbic Acid-2-phosphate protective effect against ROS on pancreatic β-cell spheroids (2019)
  • 著者名/発表者名: Dina Myasnikova, Binbin Zhang Molino, Junji Fukuda
  • 学会等名: TERMIS-WC
  • 国際学会