| 研究実績の概要 |
Last year, I have tried to finish my research project in the interaction of environmental chemicals and thyroid hormones (TH) with the nuclear receptor (thyroid hormone receptor and estrogen receptor) or cell surface receptor (integrin and GPER1/GPR30) that regulate cell proliferation, migration, neuritogenesis, and synaptogenesis. The results revealed that thyroid hormones induced cell migration and proliferation through TRα-dependent and TR-independent pathways. The thyroid hormone-induced cell migration and proliferation through the activation of Grb2 leads to activate the TR-independent pathways. In addition, environmental chemicals such as isoflavones or gadolinium-based contrast agents (GBCAs) accelerate cell migration through GPER1 or integrin activation, which leads to activate FAK/PI3K/Akt/Rho-GTPase pathways that induced F-actin re-arrangement. TH and isoflavones also induced dendritogenesis and synaptogenesis in Purkinje cells via different mechanism pathways, TRα and ERα, respectively. Cell fractionation assays showed that isoflavones induced localization of SNARE protein to the membrane and dendritic fraction, indicate the synapse formation. Isoflavones and TH also increased the expression level of both pre- and post-synaptic proteins. Now, I am preparing several manuscripts to publish my research results. We plan to publish our results in several international journals.
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