| 研究課題/領域番号 |
18K04905
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| 研究機関 | 北陸先端科学技術大学院大学 |
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研究代表者 |
ビヤニ マニッシュ 北陸先端科学技術大学院大学, 先端科学技術研究科, 客員教授 (00599780)
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| 研究分担者 |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
山下 啓子 北海道大学, 大学病院, 教授 (70332947)
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| 研究期間 (年度) |
2018-04-01 – 2021-03-31
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| キーワード | Peptide aptamer / Voltammetric sensor / Brest Cancer / Cathepsin E |
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| 研究実績の概要 |
A new scheme is designed to achieve the objectives based on voltammetric approach. For this, sequences of two peptides are optimized and synthesized.
1. CatE binding peptide aptamer
2. CatE substrate peptide
Next, in order to integrate both the peptides onto the surface of voltammetric sensor in functionally active form, a linker peptide chain composed of alpha-strand property (18 amino acids) and PEG-2000 is designed and evaluated.
As a result, detection limit of model target (amyloid beta42) could be increase as low as 4 nM using voltammetric DEPSOR sensor and disposable screen printed electrochemical chips.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
In order to make a simple POCT sensor (demand from one of the co-investigator in this project from medical-side), an alternative plan by utilization of simple sensing surface of voltammetric approach is designed and that's bit delayed the scheduled experimental plan.
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| 今後の研究の推進方策 |
Both the peptides (#1 and #2) with alpha-strand chain will be custom synthesized and coupled with nanoparticles for electrochemical analysis using DEPSOR. In the first stage, a recombinant Cathepsin E will be measured and limit of detection will be measured. After achieving a desired sensitivity, the scheme will be demonstrated for clinical sample obtained by one of the co-investigator from hospital.
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| 次年度使用額が生じた理由 |
The budget will be utilized to purchase the reagents and to hire a researcher to perform voltammetric experiment. Some amount will be used for travel too.
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