| 研究課題/領域番号 |
18K04905
|
|---|
| 研究機関 | 北陸先端科学技術大学院大学 |
|---|
研究代表者 |
ビヤニ マニッシュ 北陸先端科学技術大学院大学, サイレントボイスセンシング国際研究拠点, 特任教授 (00599780)
|
|---|
| 研究分担者 |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
山下 啓子 北海道大学, 大学病院, 教授 (70332947) [辞退]
|
|---|
| 研究期間 (年度) |
2018-04-01 – 2023-03-31
|
| キーワード | Peptide aptamer / Voltammetric sensor / Brest Cancer / Cathepsin E |
|---|
| 研究実績の概要 |
A silicon-based FET sensor is developed to measure expression-level of Cathepsin E (CatE). This was achieved by immobilizing the CatE specific substrate peptide onto the surface of FET followed by measuring the cleavage of CatE using a palm-sized portable FET device (termed FETSOR). The measurement conditions for the working of biosensor were optimized at pH 7.4 and 0.01X PBS and we could successfully differentiate between the presence and absence of 2.3 pmol CatE’s activity to cleave the substrate peptide. The overall charge on the peptide at pH 7.4 is +8.51 and after the cleavage it reduces to +3.61 which was confirmed by decrease in the gate voltage measurement by 0.05V. Therefore, we successfully demonstrated and evaluated the utility of FETSOR for the measurement of CatE.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
We are continuously searching a collaborator for providing clinical samples after the pre-retirement of Prof Yamashita from Hokkaido University.
|
| 今後の研究の推進方策 |
First, we will find a collaborator in some hospital for providing the clinical samples. Then, we will arrange to install the FETSOR system in the hospital for clinical sample testing and evaluation.
|
| 次年度使用額が生じた理由 |
Budget will be utilized to conduct the clinical sample testing and evaluation.
|
