| 研究課題/領域番号 |
18K04905
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| 研究機関 | 北陸先端科学技術大学院大学 |
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研究代表者 |
ビヤニ マニッシュ 北陸先端科学技術大学院大学, 共創的国際研究推進本部, 特任教授 (00599780)
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| 研究分担者 |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
山下 啓子 北海道大学, 大学病院, 教授 (70332947) [辞退]
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| 研究期間 (年度) |
2018-04-01 – 2023-03-31
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| キーワード | Peptide aptamer / Voltammetric sensor / Brest Cancer / Cathepsin E / Portable FET |
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| 研究実績の概要 |
A silicon-based FET sensor is developed to measure expression-level of Cathepsin E (CatE). This was achieved by immobilizing the CatE specific substrate peptide onto the surface of FET followed by measuring the cleavage of CatE using a palm-sized portable FET device (termed FETSOR). The measurement conditions for the working of biosensor were optimized at pH 7.4 and 0.01X PBS and we could successfully differentiate between the presence and absence of 2.3 pmol CatE’s activity to cleave the substrate peptide. The overall charge on the peptide at pH 7.4 is +8.51 and after the cleavage it reduces to +3.61 which was confirmed by decrease in the gate voltage measurement by 0.05V. Therefore, we successfully demonstrated and evaluated the utility of FETSOR for the measurement of CatE.
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| 備考 |
We conclude the project with the achievement of developing a portable peptide-based FET sensor for a simple and rapid detection of breast cancer biomarker Cathepsin E.
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