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2020 年度 実施状況報告書

ATP-dependent coupling of pre- and post-synaptic organelles movements at central synapses and its implication in synaptic plasticity and transmission

研究課題

研究課題/領域番号 18K06494
研究機関沖縄科学技術大学院大学

研究代表者

Guillaud Laurent  沖縄科学技術大学院大学, 細胞分子シナプス機能ユニット, 研究員 (90596222)

研究期間 (年度) 2018-04-01 – 2022-03-31
キーワードLiquid phase separation / Synapse / Mitochondria / ATP / Neurodegeneration
研究実績の概要

For the third year my research project has progressed according to plan but with a slight delay due to my move to a new research unit. However this move allowed me to also expand my work to motor neurons and related neurodegenerative pathologies.
1) I have now completed the in vitro analysis of 6 proteins involved in Parkinson and Alzheimer diseases and quantified the number and size of their condensates at 37C and in the presence or absence of ATP. I also determined the threshold concentration of ATP required to prevent liquid phase separation of each of these proteins. 2) I have also performed liquid phase separation in vitro assay on 2 additional proteins implicated in ALS disease, and demonstrated that they also undergo ATP-dependent condensation/decondensation at 37C. 3) I have also performed preliminary experiments on cultured motor neurons (derived from human iPSC and/or mouse ES cells) and show that cytosolic GFP liquid phase separation follow the same mitochondria/ATP-dependent mechanism as I previously reported in giant glutamatergic terminals, suggesting a common and conserve LPS regulation mechanism among different neuronal populations. 4) Additionally I also analyzed LPS in cultured human glutamatergic neurons (derived from iPSCs) using FRAP, as well as quantified and compared ATP concentration in normal and Alzheimer cultured neurons by in vitro luminescence assay. 5) I have written and prepared the initial draft of the manuscript

現在までの達成度 (区分)
現在までの達成度 (区分)

3: やや遅れている

理由

As I moved to a different unit at the end of 2019, my work has been slightly delayed. In addition I have also started to collect new data on different type of neurons (derived from human iPSCs or mouse ESCs) as well as on several proteins involved in ALS disease.

今後の研究の推進方策

My next research plan is to increase the number of data on motor neurons cytosolic phase separation and in vitro data on additional proteins involved in ALS or SMA.
Lastly I am planning to submit the manuscript in the first half of 2021.

次年度使用額が生じた理由

Due to my move to a new research unit and the delay in the preparation of the manuscript and the collection of additional and new set of data, I have requested carry over of my remaining budget to 2021.
the remaining budget will be used to cover the cost of reagents need for the revision of the manuscript and for the publication fee.

  • 研究成果

    (2件)

すべて 2020

すべて 雑誌論文 (2件)

  • [雑誌論文] Anterograde Axonal Transport in Neuronal Homeostasis and Disease2020

    • 著者名/発表者名
      Guillaud Laurent、El-Agamy Sara Emad、Otsuki Miki、Terenzio Marco
    • 雑誌名

      Frontiers in Molecular Neuroscience

      巻: 13 ページ: NA

    • DOI

      10.3389/fnmol.2020.556175

  • [雑誌論文] Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core2020

    • 著者名/発表者名
      Mohamed Mahmoud Shaaban、Hazawa Masaharu、Kobayashi Akiko、Guillaud Laurent、Watanabe-Nakayama Takahiro、Nakayama Mizuho、Wang Hanbo、Kodera Noriyuki、Oshima Masanobu、Ando Toshio、Wong Richard W.
    • 雑誌名

      Biomaterials

      巻: 256 ページ: 120198~120198

    • DOI

      10.1016/j.biomaterials.2020.120198

URL: 

公開日: 2021-12-27  

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