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2019 年度 実施状況報告書

魚類の給餌を最適化する新たなアプローチ:オートファジーによる生体防御機構の解明

研究課題

研究課題/領域番号 18K14520
研究機関愛媛大学

研究代表者

Mohapatra Sipra  愛媛大学, Kyushu University, Researcher (80715441)

研究期間 (年度) 2018-04-01 – 2021-03-31
キーワードAutophagy / Hexokinase / estrogen / Liver / Fish / estrogen receptor
研究実績の概要

I have characterized the HKII, III and IV profiles in Red Sea bream liver and confirmed their roles in liver autophagy regulation. Fasting and E. trade challenge experiment using both medaka and Red Sea bream, showed that several autophagy gene were significantly altered. I also isolated a 5KB promoter sequence of HKII from both fish species and found that, 17b-estradiol significantly increased the HKII promoter activity in vitro. Later, using two Estrogen receptor Knockout medaka line and CHIP analysis it was confirmed that HKII is a direct target of estrogen receptor. These data cumulatively suggests that, HKII is a prime candidate of sex biased liver autophagy in fish and can be a potential marker for ecofriendly aquaculture in future.

現在までの達成度 (区分)
現在までの達成度 (区分)

1: 当初の計画以上に進展している

理由

Earlier, I planned to isolate HKII, HKIII and HKIV from Red Sea bream and analyze their tissue distribution and cellular localization. I also planned to establish primary hepatocyte culture method, perform serum and glucose starvation under various hexokinase over expression and knockodown condition in vitro, conduct promoter analysis invitro using mammalian cell line. comparative invivo starvation and infection experiment using medaka and Red Sea bream were also planned. I have successfully finished all the planned experiment at least three times and analyzed the data.
Additionally I performed indepth analysis on sex biased autophagy and estrogen regulation and published a peer reviewed research article. I have also generated HKII knockout medaka which will be helpful to analyze the downstream cascade of HKII regulation in fish,

今後の研究の推進方策

I will decipher the downstream genes of HKII pathway using chromatin immunoprecipitation and other relevant analysis both invitro and invivo. I will also look into the hepatocyte mitochondria and get an idea about the mitophagy and HKII association. If necessary, I will investigate several other autophagy related candidates to confirm the prospects of autophagy in aquaculture management.

次年度使用額が生じた理由

During the initial period (since November 2018- May 2019, I was on maternity and child care leave, which affected my timely execution of Planned research. Hence I got delayed by few months. I will use the money in this fiscal year

  • 研究成果

    (4件)

すべて 2020 2019

すべて 雑誌論文 (2件) (うち国際共著 2件、 査読あり 2件、 オープンアクセス 1件) 学会発表 (2件) (うち国際学会 2件、 招待講演 2件)

  • [雑誌論文] Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver2020

    • 著者名/発表者名
      Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Kayoko Ohta, Yoshitaka Nagahama, Kohei Ohta
    • 雑誌名

      Cell Death & Differentiation

      巻: NA ページ: NA

    • DOI

      https://doi.org/10.1038/s41418-020-0567-3

    • 査読あり / 国際共著
  • [雑誌論文] Estrogen receptor β2 oversees germ cell maintenance and gonadal sex differentiation in medaka, Oryzias latipes2019

    • 著者名/発表者名
      2.Tapas Chakraborty, Sipra Mohapatra, Lin yan Zhou, Takahiro Matsubara, Taisen Iguchi, Yoshitaka Nagahama
    • 雑誌名

      Stem Cell Reports

      巻: 13 ページ: 419-433

    • DOI

      10.1016/j.stemcr.2019.07.013

    • 査読あり / オープンアクセス / 国際共著
  • [学会発表] Sex and sex steroids are eminent regulators of fish autophagy2019

    • 著者名/発表者名
      Sipra Mohapatra, Tapas Chakraborty, Takahiro Matsubara, Kohei Ohta
    • 学会等名
      10th International Conference on Fisheries and Aquaculture, Toronto, Canada
    • 国際学会 / 招待講演
  • [学会発表] Comparative transcriptomics and Edwardsiellosis detection in fish: a study using medaka and Red Sea bream2019

    • 著者名/発表者名
      Tapas Chakraborty, Sipra Mohapatra, Sonoko Shimizu, Takahiro Matsubara, Kiyoshi Naruse, Kohei Ohta
    • 学会等名
      10th International Conference on Fisheries and Aquaculture, Toronto, Canada
    • 国際学会 / 招待講演

URL: 

公開日: 2021-01-27  

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