|
During these last two years we have:
1. studied the methionine adenosyl transferase and exploited the catalytic promiscuity of this enzyme to synthetize biochemically synthetic analogues of SAM. 2. Establish a HPLC method to detect the formation of the SAM analogues and purify them. 3. Study three different bacterial Methyltransferases scaffold as potential starting point for our engineering study. 4. We established a UPLC-MS method to run kinetics assays for the MTases. 5. We defined the kinetics parameters for the synthetic cofactors
Overall the data collected in this period of time allowed us to write a manuscript (in preparation) and collect enough data for a second one.
|
