| 研究実績の概要 |
Defects in circadian rhythms are related to health problems, such as obesity, cancer, and depression, and understanding how RNA translation is regulated is important for developing new treatments. We used ribosome profiling to understand RNA translation in liver over a 24-h period and found that ribosomes bound to upstream open reading frames (uORFs) in circadian mRNAs repress mRNA translation. Mutation of the uORF in Per2 significantly reduced sleep in mutant mice compared to that of wild-type littermates. In the Akira lab, we used ribosome profiling to understand translation in wild-type and Regnase mutant hepatocytes, and in the Standley lab, we used RNA structure folding and machine learning to predict candidate Regnase target mRNAs.
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