| 研究課題/領域番号 |
18K14787
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| 研究機関 | 東京大学 |
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研究代表者 |
WONG SHUKUAN 東京大学, 大気海洋研究所, 海洋科学特定共同研究員 (60808400)
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| 研究期間 (年度) |
2018-04-01 – 2022-03-31
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| キーワード | Ice nucleation activity / Sea Surface Microlayer |
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| 研究実績の概要 |
Ice-nucleating bacteria have the ability to freeze at warmer temperatures with some as warm as -2 Celcius. We had isolated 157 bacterial strains from the sea surface microlayer,seawater and seafoam. The ice nucleation activity of the isolated bacterial strains were tested using the dropdown freezing assay. A total of 67 bacterial isolates had been tested for their ice nucleating activities using the freeze-drop test. 57 isolates showed no ice-nucleation potential; 54 isolates froze at -20 Celcius, the temperatures where the negative control started to freeze while three isolates froze at temperatures lower than the negative control. Three isolates were ice-nucleation positive at temperatures of -10to-11 Celcius.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
Due to maternity/childcare leave and current coronavirus conditions, this research had been left/stalled at the stage it was during the last report.
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| 今後の研究の推進方策 |
As mentioned in the previous report, we will continue to test the ice nucleating ability of the remaining 90 isolates.
Once, we have identified and obtained positive ice nucleating bacterial strains from our environmental samples, we will be able to proceed with
the next step in streamlining the PCR primers and conditions for use with environmental DNA samples.
If we succeed in amplifying the environmental samples using the PCR
primers and conditions tested above, the amplified environmental samples will be sequenced using next generation sequencer to identify the INA
bacteria more efficiently at a larger scale.
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| 次年度使用額が生じた理由 |
The balance of the funding will be brought forward to continue our experiments on the remaining 90 isolates as well as developing new primer sets
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