研究課題
We revealed that the organizational principle of dendritic spines differs according to the regions of the brain. For example, in CA1 proximal stratum radiatum, cortex and cerebellum we revealed that the total area of the postsynaptic density increases linearly with dendritic diameter. However, similar trend was not observed for the dendrites in striatum and stratum lacunosum moleculare. Glutamate uncaging experiment revealed that neck length is more influential than head volume in determining the magnitude of voltage change at the soma in the CA1 proximal radiatum.
2: おおむね順調に進展している
We were able to obtain new data regarding the scaling of the total synaptic content(the area of the postsynaptic density) in the dendrites with the dendritic diameter. In addition, we found that the ratio of PSD area to neck length was not correlated with dendritic diameter and were rather similar between dendrites of different diameters. In addition to the FIB/SEM data, we have also incorporated glutamate uncaging data in this experiment regarding the influence of spine head and spine neck in the determining the magnitude of voltage change at the soma.The proposed experiments progressed smoothly and the scientific content and the findings of this study is novel. Currently I am working a manuscript for this project to be submitted to scientific journals.
At present I am writing a manuscript compiling the data of this research. The major findings of this project and the storyline of the manuscript is clear. The study so far focused on the organization of dendritic spines in wild-type mouse. Currently the analysis of dendritic spine structure in the disease mouse model is also underway to see how the dendritic spine structure changes in pathological conditions. Moreover, in future, in addition to observing the morphology of dendritic spines I am also interested to study the organization of intracellular structures such as endoplasmic reticulum and Mitochondria in the dendrites and spines both in the wild-type and mutant mouse. Also, I am interested to analyze if the axon-coupled spines are the structural features of engram , memory spines.
In this project, during the course of experiment and data analysis we encountered some new data concerning the relationship between dendritic diameter and the synapse content. Furthermore, we also found out that the spine morphology is altered in Parkinson`s model mouse. Although the analysis is near to completion we would like to examine this issue in more detail. For this reason, there was some remaining amount of grant this year and not all the money was used up in the previous fiscal year. The remaining amount of money will be used for additional data analysis.
すべて 2020 2019
すべて 雑誌論文 (3件) (うち国際共著 3件、 査読あり 3件、 オープンアクセス 2件) 学会発表 (2件) 図書 (2件)
BRAIN
巻: 143 ページ: 249-265
doi:10.1093/brain/awz380
Human Molecular Genetics
巻: 28 ページ: 1894-1904
doi: 10.1093/hmg/ddz030
eNeuro
巻: 6 ページ: 1-22
doi: 10.1523/ENEURO.0427-18.2019.
