| 研究実績の概要 |
Mycobacteria infection in host cells triggers a dynamic cascade of altered gene expression in both interacting organisms. To elucidate the functional role of ESX-5 secreted PE/PPE proteins in tuberculosis, two BCG deletion mutants, ppe26 or ppe27, were generated by mutagenesis. Macrophage cells were subjected to infection with wild-type and two mutated BCG variants. Dual RNA-seq was performed to unveil the altered gene expression both in the bacteria and infected host cells. Released cytokines were measured by multiplexed bead-based cytokine assay. The overall results from this study suggested the immunosuppressive role of PPE26 and PPE27. This confers the opportunity of manipulating PPE26/27 protein as potential candidate for drug intervention or vaccine development.
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