| 研究実績の概要 |
This proposal aims to establish customized NGS panels to detect circulating tumor DNA (ctDNA) from various solid tumors. Two panels were established: JFCR cfDNA screening panel for cancer screening and JFCR cfDNA actionable mutation panel for detection of variants with therapeutic implication.
With 2 commercial cfDNA controls, these panels detected mutations with limit of detection at 0.2%. The correlation r2 between the NGS data and control is 0.95.
JFCR screening panel is a 33 cancer gene panel, designed after assessing mutation profiles from TCGA and COSMIC databases. ctDNA detection rate varies among solid cancers and stages, range 50-67%.
JFCR cfDNA actionable mutation panel is a 59 gene panel that covers actionable mutations, CNV and fusions. The average ctDNA detection rate is 79%.
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