| 研究課題/領域番号 |
18K16577
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| 研究機関 | 筑波大学 |
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研究代表者 |
プエンテス サンドラ 筑波大学, システム情報系, 助教 (00725765)
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| 研究期間 (年度) |
2018-04-01 – 2023-03-31
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| キーワード | Stroke intervention / Cell therapy / Mesenchimal stem cells / Microglial polarization |
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| 研究実績の概要 |
During the last year, the progress of the research was very delayed due to COVID-19. The main focus was based on blinded cell counting which was confirmed by more than one investigator and checked by a non-blinded member in order to ensure the reliability of the cell counting itself.
Samples were randomly double checked and in some cases, also re-checking of the stained samples under the microscope was performed to ensure that the collected data from pictures was actuall cell staining and not immunofluorescence-related artifacts.
The initial collected data for animal status (physiological data) was also organized and analysis is undergoing. There was consideration about collecting more information related to the animal status during the procedures in order to ensure that particular conditions did not affected the outcome of each group (note that on experiment day, two or three different injection contents were prepared and the animals operated the previous day were randomized after ensuring they fulfilled the inclusion criteria (Longa score and general status)).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
The COVID 19 situation delayed the use of animal facilities and the collection of human samples as well. Additionally, the disponibility of personel is reduced and access to facilities is well controlled in order to avoid dangerous situations regarding to COVID 19 spread. These new organization of facilities and resources impose a challenge since coordination with other inversigators sharing the same environment has become difficult and time consuming.
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| 今後の研究の推進方策 |
During the FY 2022, we plan to add the in vitro experiments and draft the paper with the initial collected data. If required, additional experiments will be added.
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| 次年度使用額が生じた理由 |
Since access to samples for cell preparation, and animal experiments were affected by the COVID-19, experiments were slowed down, and the amount of desired experiments couldn't be performed. The remaining budget relates to experiments that were delayed.
The remaining budget will be focused on supporting in vitro experiments, adding more animal samples and if possible covering part of publication fees as well.
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