肝癌に対する複合的免疫遺伝子治療:樹状細胞機能増強による免疫監視システム再構築

2009 年度 研究成果報告書

• 研究課題/領域番号: 19390200
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 消化器内科学
• 研究機関: 香川大学
• 研究代表者: 出口 章広 香川大学, 医学部, 助教 (30380174)
• 連携研究者: 正木 勉 香川大学, 医学部, 教授 (30335848)
• 研究期間 (年度): 2007 – 2009
• キーワード: 肝癌 / 肝硬変 / サイトカイン / 樹状細胞

研究概要

1.肝癌における血管新生分子の発現
非癌組織、肝癌において増強していた血管新生分子はFGF-βあるいはIL-8である。正常肝細胞株と比較して肝癌細胞株で増強していたのもFGF-βあるいはIL-8であった。
2.肝癌におけるサイトカイン分子の発現
肝癌において増強していたサイトカイン分子は、activin, CD40,FGF,Glut,IL17 and FGF-・.である。
3.肝癌におけるマイクロRNAのプロファイリング
マイクロRNA-527は肝癌において極めて減弱しており、そのターゲット遺伝子はglypican-3である。

研究成果

• [雑誌論文] High concentration of lactic dehydrogenase in small volumes of ascites (2010)
  • 著者名/発表者名: Izuishi K, Masaki T, Suzuki Y
  • 雑誌名: J Gastrointestin Liver Dis. (1) ページ: 105-6
  • 査読あり
• [雑誌論文] The close relationship between life-threatening breathing disorders and urine storage dysfunction in multiple system atrophy. (2010)
  • 著者名/発表者名: Deguchi K, Masaki T, 7others (last)
  • 雑誌名: J Neurol. ページ: 1-6
  • 査読あり
• [雑誌論文] Operative management of solitary intra-abdominal recurrence of hepatocellular carcinoma (2010)
  • 著者名/発表者名: Sano T, Izuishi K, Takebayashi R, Masaki T, Suzuki Y
  • 雑誌名: Surgery 147(2) ページ: 311
  • 査読あり
• [雑誌論文] Cytomegalovirus-associated Acute Gastric Mucosal Lesion in an Immunocompetent Host (2009)
  • 著者名/発表者名: Himoto T, Masaki T, 13 others (11)nd
  • 雑誌名: Intern Med 48 ページ: 1521-1524
  • 査読あり
• [雑誌論文] MicroRNA and hepatocellular carcinoma (2009)
  • 著者名/発表者名: Masaki T
  • 雑誌名: Hepatol Res 39(8) ページ: 751-2
  • 査読あり
• [雑誌論文] Education and imaging. Hepatobiliary and pancreatic:Isolated diaphragmatic metastasis from hepatocellular carcinoma (2009)
  • 著者名/発表者名: Sano T, Izuishi K, Takebayashi R, Kushida Y, Masaki T, Suzuki Y
  • 雑誌名: J Gastroenterol Hepatol 24 ページ: 1475
  • 査読あり
• [雑誌論文] Paraneoplastic necrotizing myelopathy in a patient with advanced esophageal cancer: An autopsied case report (2009)
  • 著者名/発表者名: Urai Y, Masaki T, 12 others (last)
  • 雑誌名: J Neurol Sci 15;280(1-2) ページ: 113-7
  • 査読あり
• [雑誌論文] Tokumitsu H, Identification and Characterization of Wolframin (2009)
  • 著者名/発表者名: Yurimoto S, Masaki T, 6 others (6)nd
  • 雑誌名: the Product of the Wolfram Syndrome Gene(WFS1), as a Novel Calmodulin-Binding Protein Biochemistry 48 ページ: 3946-3955
  • 査読あり
• [雑誌論文] Hepatic von Meyenburg complex:a triggre of severe portal hypertension (2009)
  • 著者名/発表者名: Yoshida S, Kurokohchi K, Ueno T, Yoshino M, Shimada M, Masaki T
  • 雑誌名: Liver Int 29 ページ: 614-615
  • 査読あり
• [雑誌論文] Authors' reply (2009)
  • 著者名/発表者名: Kinekawa F, Masaki T, 10 others (2nd)
  • 雑誌名: J Gastroenterol 44(3) ページ: 237
  • 査読あり
• [雑誌論文] Immunohistochemically detected expression of p27(Kip1) and Skp2 predicts survival in patients with intrahepatic cholangiocarcinomas (2009)
  • 著者名/発表者名: Hashimoto N, Masaki T, 20others (7)nd
  • 雑誌名: Ann Surg Oncol. 16(2) ページ: 395-403
  • 査読あり
• [雑誌論文] Clinical characteristics of patients with hepatitis C virus-related chronic liver disease seropositive for anticentromere antibody (2009)
  • 著者名/発表者名: Himoto T, Masaki T, Deguchi A, 9others (5, 7)thnd
  • 雑誌名: Dig Dis Sci 54(2) ページ: 360-8
  • 査読あり
• [雑誌論文] Modulating effect of the PI3-kinase inhibitor LY294002 on cisplatin in human pancreatic cancer cells (2008)
  • 著者名/発表者名: Fujiwara M, Izuishi K, Sano T, Hossain MA, Kimura S, Masaki T, Suzuki Y
  • 雑誌名: J Exp Clin Cancer Res 225;27 ページ: 76
  • 査読あり
• [雑誌論文] Annexin A2 expression and phosphorylation are up-regulated in hepatocellular carcinoma (2008)
  • 著者名/発表者名: Mohammad SH, Yoneyama H, Deguchi A, Himoto T, Masaki T, 20 others (12th, last author)
  • 雑誌名: Int J Oncol 33 ページ: 1157-1163
  • 査読あり
• [雑誌論文] How many cytological examinations should be performed for the diagnosis of malignant biliary stricture via an endoscopic nasobiliary drainage tube? (2008)
  • 著者名/発表者名: Uchida N, Masaki T, 5 others (4th)
  • 雑誌名: J Gastroenterol Hepatol 23(10) ページ: 1501-4
  • 査読あり
• [雑誌論文] Esophageal function worsens with long duration of diabetes (2008)
  • 著者名/発表者名: Kinekawa F, Masaki T, 10 others (last author)
  • 雑誌名: J Gastroenterol 43 ページ: 338-344
  • 査読あり
• [雑誌論文] Outer arm fucosylation of N-glycans increases in sera of hepatocellular carcinoma patients (2008)
  • 著者名/発表者名: Tanabe K, Deguchi A, 6 others (2nd)
  • 雑誌名: Biochem Biophys Res Commun 374 ページ: 219-225
  • 査読あり
• [雑誌論文] A combination therapy of ethanol injection and radiofrequency ablation under general anesthesia for the treatment of hepatocellular carcinoma (2008)
  • 著者名/発表者名: Kurokohchi K, Yoneyama H, Deguchi A, Masaki T, Himoto T, 6 others (4, 5)ththh
  • 雑誌名: World J Gastroenterol 14 ページ: 2037-2043
  • 査読あり
• [雑誌論文] ndrd Comparative study of the effects of percutaneous ethanol injection and radiofrequency ablation in cases treated with a straight or expandable electrode (2007)
  • 著者名/発表者名: Kurokohchi K, Deguchi A, Masaki T, Yoneyama H, Himoto T, 4 others
  • 雑誌名: Oncol Rep 18 ページ: 1275-1279
  • 査読あり
• [雑誌論文] A double filtering method for measuring the translational velocity of fluorescently stained cells (2007)
  • 著者名/発表者名: Yasokawa T, Ishimaru I, Kuriyama S, Masaki T, Takegawa K, Tanaka N
  • 雑誌名: Appl Phys Lett 91 ページ: 131116(1)-131116(3)
  • 査読あり
• [雑誌論文] thth Efficacy of zinc administration in patients with hepatitis C virus-related chronic liver disease (2007)
  • 著者名/発表者名: Himoto T, Deguchi A, Masaki T, 9 others (4, 8)
  • 雑誌名: Scand J Gastroenterol 42 ページ: 1078-1087
  • 査読あり
• [雑誌論文] ndrd Successful treatment of hypovascular advanced hepatocellular carcinoma with lipiodol-targetting intervention radiology (2007)
  • 著者名/発表者名: Kurokohchi K, Deguchi A, Masaki T, Himoto T, Yoneyama H, 7 orhers (2, 3)
  • 雑誌名: World J Gastroenterol 13 ページ: 4398-4400
  • 査読あり
• [雑誌論文] rdthVitamins K2, K3 and K5 exert antitumor effects on established colorectal cancer in mice by inducing apoptotic death of tumor cells (2007)
  • 著者名/発表者名: Ogawa M, Deguchi A, Masaki T, 5 others (3, 5)
  • 雑誌名: Int J Oncol 31 ページ: 323-331
  • 査読あり
• [雑誌論文] Utility of pancreatic duct brushing for diagnosis of pancreatic carcinoma (2007)
  • 著者名/発表者名: Uchida N, Masaki T, 8 Others (6nd)
  • 雑誌名: J Gastroenterol 42 ページ: 657-662
  • 査読あり
• [雑誌論文] Usefulness of single and repetitive percutaneous transhepatic gallbladder aspiration for the treatment of acute cholecystitis. (2007)
  • 著者名/発表者名: Tsutsui K, Masaki T, 11 Others (11th)
  • 雑誌名: J Gastroenterol 42 ページ: 583-588
  • 査読あり
• [雑誌論文] A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting mach-zehnder interferometer (2007)
  • 著者名/発表者名: Yasokawa T, Masaki T, 5 others (5th)
  • 雑誌名: Optical Review 14 ページ: 161-164
  • 査読あり
• [雑誌論文] Abundance of microRNA target motifs in the 3'-UTRs of 20527human genes (2007)
  • 著者名/発表者名: Iwama H, Masaki T, Kuriyama S.
  • 雑誌名: FEBS Lett. 1;581(9) ページ: 1805-10
  • 査読あり
• [雑誌論文] Technique for measuring the rotational velocity of a single cell. (2007)
  • 著者名/発表者名: Yasokawa T, Masaki T, 6 others (6th)
  • 雑誌名: Appl Phys Lett 90(5)no.051103
  • 査読あり
• [雑誌論文] thth Sequential assessment of the intrahepatic expression of epidermal growth factor and transforming growth factor-beta1 in hepatofibrogenesis of a rat cirrhosis model (2007)
  • 著者名/発表者名: Kuriyama S, Deguchi A, Masaki T, 12 others (8, 13)
  • 雑誌名: Int J Mol Med ページ: 317-324
  • 査読あり
• [雑誌論文] (2007)
  • 著者名/発表者名: Yamamoto K, Masaki T, 6 others (6th)
  • 雑誌名: Appl Phys Lett 90 ページ: 021110-1-021110-3
  • 査読あり
• [雑誌論文] Identification of c-Yes expression in the nuclei of hepatocellular carcinoma cells: Involvement in the early stages of hepatocarcinogenesis (2006)
  • 著者名/発表者名: Nonomura T, Masaki T, Himoto T, 9 others (2nd)
  • 雑誌名: Int J Onco1 ページ: 105-111
  • 査読あり
• [雑誌論文] Displacement measurement of the depth migration of transparent cells (2006)
  • 著者名/発表者名: Yoshida M, Masaki T, 7others (6th)
  • 雑誌名: Appl Phys Letts 89(24) ページ: 241102-1-241102-3
  • 査読あり
• [雑誌論文] Three-dimensional phase-contrast imaging of single floating cells (2006)
  • 著者名/発表者名: Kobayashi H, Masaki T, 7others (6th)
  • 雑誌名: Appl Phys Letts 89(24) ページ: 241117-1-241117-3
  • 査読あり
• [雑誌論文] Imaging technology of three dimensional distribution for sugar chain on single living cell-membrane (2006)
  • 著者名/発表者名: Yamamoto K, Masaki T, 9 others (11th)
  • 雑誌名: Proceedings of SPIE-The International Society for Optical Engineering 6374no.63740L
  • 査読あり
• [雑誌論文] Successful treatment of large-size advanced hepatocellular carcinoma by transarterial chemoembolization followed by the combination therapy of percutaneous ethanol-lipiodol injection and radiofrequency ablation (2006)
  • 著者名/発表者名: Kurokohchi K, Masaki T, Yoneyama H, 9 others (9)th
  • 雑誌名: Oncol Rep 16 ページ: 1067-1070
  • 査読あり
• [雑誌論文] Multidisciplinary treatment of patients with hepatocellular carcinoma (2006)
  • 著者名/発表者名: Masaki T, Morishita A, Kurokohchi K, Kuriyama S
  • 雑誌名: Expert Rev Anticancer Ther 6 ページ: 1377-1384
  • 査読あり
• [雑誌論文] Attitudinal manipulation of an optically trapped bacillary probe by controlling the distance between focal points for local dosing in cells. (2006)
  • 著者名/発表者名: Yasokawa T, Masaki T, 7 others (5th)
  • 雑誌名: Appl Phys Lett 89,(13) ページ: 131107-1-131107-3
  • 査読あり
• [雑誌論文] Variable phase-contrast fluorescence spectrometry for fluorescently stained cells (2006)
  • 著者名/発表者名: Inoue Y, Masaki T, 7 others (5th)
  • 雑誌名: Appl Phys Lett 89,(12) ページ: 121103-1-121103-3
  • 査読あり
• [雑誌論文] Activation of c-Yes in hepatocellular carcinoma: A preliminary study (2006)
  • 著者名/発表者名: Feng H, Masaki T, Deguchi A, 14 others (2nd, 7th)
  • 雑誌名: World J Gastroenterol 12 ページ: 5743-5745
  • 査読あり
• [雑誌論文] Further resolution of alpha-fetoprotein glycoforms by two-dimensional isoelectric focusing and lectin affinity electrophoresis (2006)
  • 著者名/発表者名: Ichikawa E, Masaki T, 5 others (5th)
  • 雑誌名: Electrophoresis 27 ページ: 3480-3487
  • 査読あり
• [雑誌論文] Use of protein array to investigate receptor tyrosine kinases activated in gastric cancer.
  • 著者名/発表者名: Jian G, Masaki T, 18 others (last)
  • 雑誌名: Int J Oncol (in press)
  • 査読あり
• [学会発表] Tumor morphology and prognosis after radiofrequency thermal ablation for small hepatocellular carcinoma (2009)
  • 著者名/発表者名: Deguchi A
  • 学会等名: 19th Conference of the APASL
  • 発表場所: Hong Kong Convention and Exhibision Centre, Hong Kong, China
  • 年月日: 20090213-20090216