| 研究課題/領域番号 |
19F19061
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| 研究機関 | 東京大学 |
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研究代表者 |
伊藤 大知 東京大学, 大学院医学系研究科(医学部), 准教授 (50447421)
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| 研究分担者 |
CHANDEL ARVIND 東京大学, 医学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2019-04-25 – 2021-03-31
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| キーワード | Anti-adhesion / Hemostatic / Injectable / Hydrogel / Biocompatible |
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| 研究実績の概要 |
Partially oxidized Dextran was grafted with Poly[2 (Dimethylamino)ethyl Methacrylate] (PDMAEMA) by ATRP to form Dex-g-PDMAEMA. Serotonin (SRT) was reacted with partially oxidized aldehyde containing Dex-g-PDMAEMA to form SRT-g-Dex-PDMAEMA, and chloride terminated poly(ethylene glycol) (Cl-PEG-Cl) was prepared by the esterification reaction. The material was characterized by the 1H NMR and FTIR. The hydrogel was prepared by mixing the aqueous solution of SRT-g-Dex-PDMAEMA and Cl-PEG-Cl. The chemically crosslinked hydrogel was obtained, by the reaction between tertiary amine group of PDMAEMA and chloride terminated PEG. The gelation time of the hydrogel was 6-10 min its basically depends on concentration of the prepolymers solution and temperature. The obtained hydrogel was showed 10-12k storage modulus, 400-600 % w/w swelling in PBS 7.4 and 600-800% swelling in pH 5. The degradation of the hydrogel was evaluated in-vitro in two different pH 7.4 and pH 5, 15-25 days takes to complete hydrogel degradation. Degradation mainly depends on the hydrogel composition and crosslinking density. In vitro cytotoxicity was performed for obtained hydrogel exhibited good cytocompatibility with NIH 3T3 cells. The In-vivo hemostatic behavior of hydrogel was evaluated using mice liver hemorrhage model. The hydrogel showed excellent hemostatic capacity in comparison to control. The hemostatic behavior of the hydrogel due to two component first formation of permanent positive charge on tertiary amine of PDMAEMA upon crosslinking and second one is pendent serotonin moiety.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The current status of project is the injectable cationic hemostatic hydrogel was successfully prepared. The initial characterization of the obtained hydrogel has done, the gelation time of the hydrogel was 6-10 min occurred. The obtained hydrogel was showed 10-12k storage modulus, and 400-600 % w/w swelling in PBS 7.4 and 600-800% swelling in pH 5 was observed. The degradation of the hydrogel was evaluated in-vitro in two different pH 7.4 and pH 5, 15-25 days takes to complete hydrogel degradation. Degradation mainly depends on the hydrogel composition and crosslinking density. In -vitro cytotoxicity was accessed by MTT assay. The obtained hydrogel exhibited good cytocompatibility. The In-vivo hemostatic behavior of hydrogel was evaluated using mice liver hemorrhage model.
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| 今後の研究の推進方策 |
In the part of future research planning, optimization of appropriate PDMAEMA grafting on the Dextran backbone and study of their effect on the crosslinking density, various rheological properties, degradation, swelling profiles in presence of various stimuli system such as pH and degrading enzymes. Evaluation of antimicrobial efficacy and In vivo cytocompatibility in rat/mice mode thereafter evaluation of postoperative antiadhesive properties in rat model.
Preparation of similar hydrogel system with different polysaccharide such as hyaluronic acid without using acrylate polymer and evaluation of their In-vivo and Invitro hemostatic, biocompatibility, antiadhesive, antimicrobial properties. Analysis of research observations and results, preparation of research article for publication.
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