| 研究課題/領域番号 |
19F19067
|
|---|
| 研究機関 | 国立研究開発法人物質・材料研究機構 |
|---|
研究代表者 |
エルサフティ シェリフ 国立研究開発法人物質・材料研究機構, 機能性材料研究拠点, 独立研究者 (60517663)
|
|---|
| 研究分担者 |
EMRAN MOHAMMED 国立研究開発法人物質・材料研究機構, 機能性材料研究拠点, 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2019-07-24 – 2021-03-31
|
| キーワード | nanosensor / fabrication / hierarchy structure |
|---|
| 研究実績の概要 |
We conducted our research in the first year for preparing the active materials that can sensitively detect the monoamine neurotransmitters (MANTs) with high selectivity. We synthesized dual doping of nitrogen (N) and phosphorus (P) doped carbon-based nanosheets. The prepared materials exhibited a meso-micro cage, high surface area, nanocarbon construction with heterogeneous surface topography and composition. The nanosensor was employed as signalling transduction element for selective monitoring of dopamine (DA) in neuronal cell line (SH-SY5Y and PC12). The best pH-sensing value was near the physiological value (pH=7), which opened the reliability of the designed nanosensor in real human samples even in-vitro, and/or in-vivo application. The nanosensor showed a highly sensitive protocol for signaling of DA at very low concentrations with detection limit of 0.0003 uM, which is lower than the real values in the human plasma (0.23uM), and the human brain (0.02-0.2uM). Therefore, our sensory protocol showed a promising approach for sensitive detection of MANTs with high selectivity. The calibration curve was presented to detect unknown concentrations of DA that existed in the real samples. The nanosensor detected the DA secreted from human neuron cell line model (SHSY-5Y and PC12) with high sensitivity and selectivity. The nanosensor based on P-, N- doped carbon materials presented a highly promising electrode surface that can detect the MANTs in the physiological levels with high sensitivity and selectivity, high biocompatibility, and low cytotoxicity.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The materials synthesis with multi cage and numerous of active centres based on metal free catalysts for selective monitoring of the monoamine neurotransmitters (MANTs) have been established so far. The proposed materials were controlled synthesis based on green synthesis methodology using glucose as carbon source, guanine as a nitrogen source, dibasic hydrogen phosphate as a phosphorus source, and sodium dodecyl sulfate as a directing agent. The synthesized materials morphology, surface nature, and chemical composition were investigated using FE-SEM, EDS-SEM, N2-adsorption-desorption isotherm, Raman Shift, and XRD. The electrochemical behaviour was studied to establish a sensitive and selective nanosensor for signalling of MANTs. The designed nanosensor showed selective monitoring of DA at the nanoscale level. The optimizing conditions of sensitivity, selectivity, and pH supporting electrolyte were examined. The human neuronal cell line model of SHSY-5Y and PC12 were used as neuronal cell line models for cell viability and biocompatibility studies. The dual doping of N-, P- doped carbon-based materials showed low cytotoxicity using CCK-8-protocol. Signalling of MANTs liberated from neuronal cells model were traced at the designed nanosensor of dual doping of N-, P- doped carbon-based materials. The designed nanosensor showed highly sensitive and selective biosensor for the detection of MANTs at low levels secreted from neuronal cell line models of SHSY-5Y and PC12.
|
| 今後の研究の推進方策 |
The controlled fabrication and calibration of a lab-on-chip (LOC) for portable monitoring of the monoamine neurotransmitters (MANTs) will be studied. The calibration conditions such as selectivity, sensitivity, and optimum operation conditions including power consumption, the power needed, and the effect of temperature will be proceeded for producing highly compatible nanosensor. The LOC will be fabricated with billions of active atomic site surfaces, and interior space caves and grooves for MANTs monitoring in living cells. In this monitoring assay of MANT-dose levels, the electrochemical and self-visualization mechanism will be based on the quantitative tracking/signaling. At optimum conditions, the LOC will be studied for monitoring of MANTs in real sample conditions such as neuronal cell line models. The LOC would be fabricated using various thin-film technology techniques such as stereolithography, and inkjet printing. The suitable fabricated method would be used to establish micro-electromechanical system (MEMS) based devices for on-site monitoring of MANTs. Optimization of indoor or outdoor online marking and identification of MANTs will be carried out using MEMS portable devices. Towards attaining the signaling transduction, we will examine LOC-electrode design at (i) low-temperature workability, reliable cycling > 100,000 cycles, at (ii) low power consumption, and at (iii) sensitive sun-light surface electrode for detection/recognition even when operated in the “open-circuit voltage” mode.
|
