| 研究実績の概要 |
The aim of this project is to design of biocompatible scintillating nanoparticles composed of scintillator (Sc) and photosensitizer (PS) for X-ray-induced photodynamic therapy for cancer treatment. In this formulation, Sc converts X-ray to light energy to activate PS, producing reactive oxygen species (ROS). To this end, terbium-doped NaYF4 (NaYF4:Tb) was fabricated as a Sc and conjugated with rose Bengal (RB) as a PS. We have successfully fabricted PS-loaded Sc nanoparticles and have completed their physicochemical characterizations, including dynamic light scattering, electrophoretic light scattering, transmission electron microscope (TEM) imaging, UV-visible absorbance measurement, and photoluminescence (PL) and X-ray radioluminescence measurement.
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| 今後の研究の推進方策 |
We will continue biological characteristics to implement our ongoing research. In the beginning of the fiscal year, we will detect ROS in the presence of X-ray. We will use DCFH-DA assay to detect generation of ROS in an X-ray dose-dependent manner. More importantly, ROS-induced DNA damage and cell death will be examined by immunofluorescence stainning and CCK-8 assay kit, respectively. Then, the best formulation will be studied in vivo to evaluate the effective tumor accumulation after intravenous administration into subcutaneous breast tumor bearing balb/c mice. The tumor accumulation as injected dose (%) will be measured by inductively coupled plasma mass spectrometry (ICP-MS). Ultimately, the tumor will be irradiated by several X-ray dose to determine dose-dependent antitumor efficacy.
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