| 研究実績の概要 |
In the last year, the expression level of key genes including PRSb , KN1, and DL was checked, using quantitative RT-PCR, in both mold-treated samples and control samples of J. prismatocarpus. Preliminary results revealed that PRSb expression is reduced in some mold-treated samples as compared to control samples. Nonetheless, the expression level of these genes among samples fluctuated in a wide range (even for control samples). Therefore, additional experiments are being carried out. In addition, a round shaped species, J. wallichianus, was used as a second control, which should have low PRSb expression. Results of PRSb expression level of mold-treated samples of J. prismatocarpus will be interpreted together with control J. prismatocarpus (flat shape) and J. wallichianus (round shape). In addition, a few other mold designs were tried, with the aim to induce ectopic margins. This experiment is currently in process.
At the same time, the condition of non-invasively examining the shape of mold treated J. prismatocarpus leaf using X-ray computed tomography scan (CT) was established. This is helpful to know the leaf shape before using sample for qRT-PCR.
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