| 研究課題/領域番号 |
19F19764
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
LAURINO Paola 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 准教授 (90812256)
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| 研究分担者 |
DINDO MIRCO 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 外国人特別研究員
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| 研究期間 (年度) |
2019-11-08 – 2022-03-31
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| キーワード | Disordered regions / AGT / protocells / Liquid phase separation |
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| 研究実績の概要 |
In this year we have mainly worked with the alanine:glyoxylate aminotransferase, because we discovered some intrinsically disordered regions on the protein surface. In detail we have performed several investigation on these regions from a bioinformatic (molecular dynamics), biochemical (library screening and production and mutants purification as well) and cellular point of view. In the meantime we are also working in the preparation of a new system to study enzymes behavior, generating enzymatically active protocells using the liquid-liquid phase separation.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The paper on the disordered regions of AGT is ready for submission and the other one of the enzymatically active protocells as well. We are just working on the final details before submission.
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| 今後の研究の推進方策 |
The project about DPAT did not give the expected results, we are still optimizing the assay and the engineering part of the protein. We are also currently working with the enzymatically active protocells , in detail we are studying the effects of enzymatic activity on the protocells environment.
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