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2019 年度 実績報告書

AHL転写因子を介した植物の器官成長を司る転写制御ネットワークの解明

研究課題

研究課題/領域番号 19F19781
研究機関国立研究開発法人理化学研究所

研究代表者

杉本 慶子  国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)

研究分担者 FAVERO DAVID  国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
研究期間 (年度) 2019-11-08 – 2022-03-31
キーワードAHL / SOB3 / petiole / PIF / transcription / growth / plants / photomorphogenesis
研究実績の概要

The AHL transcription factors are known to repress hypocotyl growth in seedlings. However, it is largely unknown how AHLs are positioned in the gene regulatory networks (GRNs) which regulate growth. I have sought to begin to answer this question by studying how AHLs regulate petiole growth in Arabidopsis. Genetic studies combined with data from RNA-seq and ChIP-seq experiments have revealed that SOB3/AHL29 and other AHLs repress petiole elongation by inhibiting the ability of PHYTOCHROME INTERACTING FACTORs (PIFs) to activate the transcription of growth-promoting genes. This led me to investigate in detail the mechanism by which SOB3 inhibits PIF-mediated activation of growth-promoting genes. Semi-quantitative western blot analysis revealed that induction of SOB3 reduces PIF4 protein levels. ChIP-qPCR further revealed PIF4 binding to target growth-promoting genes is reduced following SOB3 induction.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

Within the last fiscal year of this project, the western blot and ChIP-qPCR experiments described above were completed. These were the final pieces of data needed to finish a paper that was resubmitted to Current Biology and then published online in this journal in March. Specifically, this paper describes how AHLs repress petiole growth by reducing PIF-mediated activation of growth-promoting genes in two different ways. First, AHLs reduce the level of PIF4 protein, likely by enhancing its degradation, as PIFs are relatively unstable in light-grown plants. Second, AHLs bind similar DNA regions as those bound by PIFs, directly inhibiting the ability of these transcription factors to bind to and activate target genes.

今後の研究の推進方策

Several different studies suggest that AHLs regulate gene transcription by serving as part of a larger DNA-protein complex. Some putative co-factors potentially important for AHL function have been identified. In order to further investigate how AHLs fit into GRNs that regulate growth, I will evaluate how these putative co-factors affect AHL-mediated transcriptional changes and growth repression. Further, I will investigate how various stimuli and transcription factors regulate expression of AHLs.

  • 研究成果

    (6件)

すべて 2020 その他

すべて 国際共同研究 (2件) 雑誌論文 (2件) (うち国際共著 2件、 査読あり 2件、 オープンアクセス 1件) 学会発表 (1件) 備考 (1件)

  • [国際共同研究] University of Potsdam(ドイツ)

    • 国名
      ドイツ
    • 外国機関名
      University of Potsdam
  • [国際共同研究] Washington State University(米国)

    • 国名
      米国
    • 外国機関名
      Washington State University
  • [雑誌論文] AT-Hook Transcription Factors Restrict Petiole Growth by Antagonizing PIFs2020

    • 著者名/発表者名
      Favero David S.、Kawamura Ayako、Shibata Michitaro、Takebayashi Arika、Jung Jae-Hoon、Suzuki Takamasa、Jaeger Katja E.、Ishida Takashi、Iwase Akira、Wigge Philip A.、Neff Michael M.、Sugimoto Keiko
    • 雑誌名

      Current Biology

      巻: 30 ページ: 1454~1466

    • DOI

      https://doi.org/10.1016/j.cub.2020.02.017

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Mechanisms regulating PIF transcription factor activity at the protein level2020

    • 著者名/発表者名
      Favero David S.
    • 雑誌名

      Physiologia Plantarum

      巻: - ページ: -

    • DOI

      https://doi.org/10.1111/ppl.13075

    • 査読あり / 国際共著
  • [学会発表] AT-hook transcription factors restrict petiole growth by antagonizing PIFs2020

    • 著者名/発表者名
      David S. FAVERO
    • 学会等名
      Japanese Society of Plant Physiologists
  • [備考] Cell Function Research Team

    • URL

      http://cellfunction.riken.jp/

URL: 

公開日: 2021-01-27  

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