| 研究実績の概要 |
In this project, we explore the challenges and opportunities of the development of a new class of versatile, massfabrication-compatible sensors in the 10 nm range. This is an ideal length scale for statistical analysis, single entity measurement and for taking advantages of unique features arising at that scale. Following the purpose of this study, we plan to compose four steps to achieve the goal.
(i) Interfacing existing gold nanoarrays with mocro- and nanofludic channels to reduce the nanoarray area, reduce the concentration of assembled biomolecules and to improve the kinetics of assemble and molecular interactions. The platform will be tested for electrochemical DNA arrays, and ion sensors composed of crown-ether modified electrochemical DNA array.
(ii) Optimizing our sensors to get a single DNA molecule per nanodot.
(iii) The optimized platform will be demonstrated for single-molecule arrays.
(iv) The device will be tested in biological environment, such as blood serum and for studying trapped CTCs.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
During the first period, we have finished the step (i) and now we are on the progress of step (ii). We have fabricated and obtained uniform and statistical distributions of nanodot arrays with diameter ranges from 10 nm to 500 nm in 1.5 mm × 1.5 mm area by using electron beam lithography after optimizing the fabrication process. For example, we used scanning electron microscope (SEM) and atomic force microscope (AFM) to characterize the nanodots array sample, which shows clear images of nanodots with diameter of 10 nm,30 nm, 50 nm, 100 nm ,200 nm.
At the same time, the ferrocene-aptamer-thiol （Fc-Aptamer-SH） electroactive molecule was drafted on the surface of gold to test the electrochemical DNA for the precursor experiment on nanodot arrays. For instance, we obtained nice voltammogram of Fc-Aptamer-SH self assembled monolayer (SAM) on gold surface, the integration of the main peek area in the voltammogram corresponds to the number of DNA molecule on the gold surface. In other words, it means a highly ordered structure corresponding to a single peak in the voltammogram can be successfully formed on the top of the gold surface. Meanwhile, the MCF-7 cancer cells have been tested in this experiment, and we are analyzing the results. Then the Fc-Aptamer-SH SAM will be drafted on the nanodot arrays in the near future.
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| 今後の研究の推進方策 |
In a short term, more microfluidic channels will be fabricated in polyetheretherketone (PEEK) with a newly purchased 3D PEEK printer to combine with our current experimental platform, to make sure our platform is more efficient. Then we will go to the next step, that we will optimize our biosensors to get single DNA per dot. After that, we will fellow our objective to accomplish our project. Meanwhile, we are writing a manuscript for publication and planning to submit an abstract for a international conference in this year.
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