| 研究課題/領域番号 |
19F19805
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| 研究機関 | 東京大学 |
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研究代表者 |
合田 圭介 東京大学, 大学院理学系研究科(理学部), 教授 (70518696)
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| 研究分担者 |
GALA DE PABLO JULIA 東京大学, 理学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2019-11-08 – 2022-03-31
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| キーワード | FT-CARS / Raman Microalgae / Euglena Starch / Carotenoid / Carbohydrate Paramylon |
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| 研究実績の概要 |
The pulse-shaping capabilities were improved and a transform-limited pulse was achieved, improving the sensitivity and signal quality for carbohydrates and carotenoids. Heavy ions mutagenesis was performed in Euglena gracilis, identifying via FT-CARS paramylon hyperaccumulating mutants, whose genetic analysis is being done. Analysis of starch accumulation in Chromochloris zofingiensis via FT-CARS was done and enzymatic analysis is now being done as a golden standard. Sorting of multiple microalgae species was achieved. Human blood signal was obtained via FT-CARS. However, leukocyte detection is still challenging with the current system sensitivity. An additional application of the FT-CARS system was identified as microplastics detection and is being researched.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Blood was detected satisfactorily, but as white blood cells could not be detected, the initial project is on hold, and thus other bio-applications are being explored until the sensitivity of the system can be improved. However, satisfactory progress is being done on other applications and the system capabilities have been improved, with the potential to detect mammalian white blood cells by the end of the project.
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| 今後の研究の推進方策 |
During the remaining of my research fellowship I will aim my efforts on 4 projects: i) Directed Evolution of Euglena gracilis: I will focus on characterizing the isolated mutants by looking at their expression of paramylon in high and low carbon media as well as genetic analysis. ii) Otimization of starch and carotenoids expression of Chromochloris zofingiensis and mutagenesis to identify astaxanthin expressing mutants. iii) in-flow FT-CARS detection for microplastics analysis. iv) Increasing sensitivity for leukocyte detection in blood. Additionally, imaging capabilities will be integrated in the system, as well as increasing bandwidth.
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