Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism

2019 年度 実績報告書

• 研究課題/領域番号: 19H00920
• 研究機関: 東京大学
• 研究代表者: キャンベル ロバート.アール 東京大学, 大学院理学系研究科(理学部), 教授 (40831318)
• 研究期間 (年度): 2019-04-01 – 2020-03-31
• キーワード: Protein Engineering / Fluorescence / Microscopy / Cell Biology

研究実績の概要

The goal of this project was to perform "Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism" by using the Grant-in-Aid for Scientific Research (A). In this fiscal year, this grant enabled us to set up the laboratory for the study mentioned above. For example, we purchased a wide-field fluorescence microscopy, which Campbell lab is using to accelerate the development of a range of optogenetic indicators.

現在までの達成度 (段落)

令和元年度が最終年度であるため、記入しない。

今後の研究の推進方策

令和元年度が最終年度であるため、記入しない。

研究成果

• [雑誌論文] Engineering genetically encoded fluorescent indicators for optical imaging of neuroscience: progress and prospects (2020)
  • 著者名/発表者名: Y. Shen, Y. Nasu, I. Shkolnikov, A. Kim and R.E. Campbell
  • 雑誌名: Neuroscience Research 巻: 未定 ページ: 未定
  • 査読あり / 国際共著
• [雑誌論文] 20 years of engineering single fluorescent protein-based biosensors (2020)
  • 著者名/発表者名: Y. Nasu, Y. Shen, L. Kramer, and R.E. Campbell
  • 雑誌名: Nat. Chem. Biol. 巻: 未定 ページ: 未定
  • 査読あり / 国際共著
• [雑誌論文] Genetically encoded fluorescent indicators for imaging intracellular potassium ions (2019)
  • 著者名/発表者名: Y. Shen, S.-Y. Wu, V. Rancic, A. Aggarwal, Y. Qian, S.-I. Miyashita, K. Ballanyi, R.E. Campbell*, and M. Dong*
  • 雑誌名: Commun. Biol. 巻: 2 ページ: 18
  • DOI: 10.1038/s42003-018-0269-2
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] A genetically encoded near-infrared fluorescent calcium ion indicator (2019)
  • 著者名/発表者名: Y. Qian (equal contribution), K.D. Piatkevich (equal contribution), B. McLarney (equal contribution), A.S. Abdelfattah, S. Mehta, M.H. Murdock, S. Gottschalk, R.S. Molina, W. Zhang, Y. Chen, J. Wu, M. Drobizhev, T.E. Hughes, J. Zhang, E.R. Schreiter, S. Shoham, D. Razansky, E.S. Boyden, and R.E. Campbell*
  • 雑誌名: Nat. Methods 巻: 16 ページ: 171-174
  • DOI: 10.1038/s41592-018-0294-6
  • 査読あり / 国際共著
• [雑誌論文] Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators (2019)
  • 著者名/発表者名: R. Molina, Y. Qian, J. Wu, Y. Shen, R.E. Campbell, T. Hughes, and M. Drobizhev
  • 雑誌名: Biophys. J. 巻: 116 ページ: 1873-1886
  • DOI: 10.1016/j.bpj.2019.04.007
  • 査読あり / オープンアクセス / 国際共著
• [学会発表] Filling the visible spectrum, and beyond, with genetically encoded fluorescent probes of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: The 10th IBRO World Congress of Neuroscience, EXCO Convention Center, Daegu, Korea
  • 国際学会 / 招待講演
• [学会発表] Engineering new protein-based biosensors to illuminate signalling and metabolism in the brain (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: World Molecular Imaging Congress, “Synthetic Biology and Brain Function”, Montreal QC
  • 国際学会 / 招待講演
• [学会発表] The legacy of camgaroo: new fluorescent spies of neural activity and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Roger Tsien Memorial Symposium, La Jolla, CA
  • 国際学会 / 招待講演
• [学会発表] Engineering protein-based biosensors for fluorescence imaging of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Department of Chemistry, National Taiwan University, Taipei, Taiwan
  • 国際学会 / 招待講演
• [学会発表] Engineering protein-based biosensors for fluorescence imaging of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Institute of Chemical Biology, Academia Sinica, Taipei, Taiwan
  • 国際学会 / 招待講演
• [学会発表] Genetically Encoded Tools [Fluorophores] and their Applications (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: LumiSTAR Opening Ceremony, National Biotechnology Research Park, Taipei, Taiwan
  • 国際学会 / 招待講演
• [学会発表] Engineering genetically-encoded fluorescent indicators for dynamic visualization of cell signaling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Quantitative Biology 2019: Dynamic Signaling in Cells and Embryos, Yantai, China
  • 国際学会 / 招待講演
• [学会発表] Filling the visible spectrum, and beyond, with genetically encoded fluorophores and indicators (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: CCMA 2019, Cytometry & Microscopy Symposium, Edmonton, AB
  • 国際学会 / 招待講演
• [学会発表] Genetically encoded fluorescent probes for multi-parameter imaging of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Advances in Fluorescent Probe and Fluorescence Imaging Modalities, 102nd Canadian Chemistry Conference and Exhibition, Quebec City, QC
  • 国際学会 / 招待講演
• [学会発表] Genetically encoded fluorophores and reporters to illuminate neuronal activity (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Neurophotonics Summer School, Cervo Brain Research Centre
  • 国際学会 / 招待講演
• [学会発表] Imaging of cell signalling with micron-scale genetically encoded biosensing beacons (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Serendipity Lab Kickoff Symposium (Half-Global), University of Tokyo
  • 国際学会 / 招待講演
• [学会発表] New genetically encoded biosensors for fluorescence imaging of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Microscopical Society of Canada 46th Annual Meeting, Vancouver, BC
  • 招待講演
• [学会発表] Fluorescent probes and proteins (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Analytical & Quantitative Light Microscopy, Marine Biological Laboratory, Woods Hole, MA
  • 国際学会 / 招待講演
• [学会発表] Genetically-encoded red and near-infrared neural activity indicators and new indicator development (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: OSA Biophotonics Congress: Optics and the Brain (New Indicators), Tucson, AZ
  • 国際学会 / 招待講演
• [学会発表] Multiplexed imaging with red-shifted optogenetic indicators (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: NIH Neuroscience Seminar Series, Bethesda, MD
  • 国際学会 / 招待講演
• [学会発表] Protein engineering and directed evolution for the creation of optogenetic indicators (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Department of Chemistry, Purdue University
  • 国際学会 / 招待講演
• [学会発表] Filling the visible spectrum, and beyond, with genetically encoded indicators of cell signalling and metabolism (2019)
  • 著者名/発表者名: Robert E. Campbell
  • 学会等名: Department of Cell Biology Seminar Series
  • 招待講演
• [学会発表] Engineering and directed evolution of protein-based tools (2019)
  • 著者名/発表者名: Yusuke Nasu, Robert E. Campbell
  • 学会等名: The 19th BIO UT
• [学会発表] Metabolismの「非」常識を検証する －近赤外蛍光乳酸センサーの開発－ (2019)
  • 著者名/発表者名: Yusuke Nasu, Robert E. Campbell
  • 学会等名: The 4th Toyota Riken meeting
• [学会発表] Development of a genetically encoded intensiometric lactate indicator iLACCO1 (2019)
  • 著者名/発表者名: Yusuke Nasu, Robert E. Campbell
  • 学会等名: ACS Fall 2019 National Meeting & Exposition
  • 国際学会
• [備考] Biomolecular Chemistry Laboratory
  • URL: https://www.chem.s.u-tokyo.ac.jp/campbell/