| 研究実績の概要 |
Immobilization of proteins onto plastic surfaces such as PMMA is primarily driven by hydrophobic interactions, that not only causes high non-specific adsorption of proteins, but also leads to protein unfolding to expose hydrophobic residues. Here, a simple and tailored method was demonstrated by exploiting the ability of air plasma to generate activated carboxyl groups on PMMA that enabled covalent coupling of proteins via EDC－NHS mediated chemistry while reducing non-specific adsorption. The rapid and scalable, yet controlled, bio-functionalization method holds promise to develop reproducible, high-throughput protein screening systems in the near future. This work was published in ACS applied materials & interfaces.
Additionally, by exploiting the benefits of microfluidics and state-of-the art fabrication techniques using 3D printing, a completely integrated, easy-to-use, hand-held, manually powered and disposable fluid processing, and biochemical assay device was developed. The potential of this prototype to serve as point-of-care testing devices was demonstrated by detecting antibodies specific to C. trachomatis infections. In the future, the inclusion of multiplexed microfluidic assay chambers within one single device, would allow detection of multiple bio-analytes from one single sample, thereby reducing both the testing time (<30 min) and the cost (< US $25 for multiple tests), for discrete at-home testing. This work was published in VIEW.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Briefly, I developed a simple air plasma enhanced surface chemistry protocol on PMMA substrates that facilitate the covalent coupling of proteins via EDC－NHS mediated chemistry with reduced non-specific adsorption. This work is important for developing bioassays with high throughput protein screening capacity. This work led to a high quality publication in ACS Applied Materials & Interfaces as first author. In addition, I also built a syringe based point-of-care testing device for detecting C. trachomatis infections. This work holds great promises for low cost diagnostic tool development and was recently published in VIEW, as first author.
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