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2019 年度 実績報告書

昆虫と細菌の内部共生を支える分子基盤の解明

研究課題

研究課題/領域番号 19J11493
研究機関北海道大学

研究代表者

JANG Seonghan  北海道大学, 農学院, 特別研究員(DC2)

研究期間 (年度) 2019-04-25 – 2021-03-31
キーワードsymbiosis / symbiont / Duox / Dual oxidase / Insect / Burkholderia
研究実績の概要

In this fiscal year, I tried to find critical genes to shape symbiosis between host insect Riptortus pedestris and its gut symbiont Burkholderia insecticola. I found one critical gene called "Duox" which encodes enzymes generating reactive oxygen species (ROS). This enzyme is well-studied in insect gut immunity originally due to a microbicidal activity of ROS, but I identified the novel role of this enzyme. Different with other insects, duox was expressed highly in the bean bug's trachea (respiratory system), and duox stabilized tracheal network by forming dityrosine network of the tracheal matrix protein. Upon duox-RNAi, tracheal network was collapsed result in a collapse of the symbiotic association. This novel finding will greatly contribute to understanding insect-bacteria symbiosis.

現在までの達成度 (区分)
現在までの達成度 (区分)

1: 当初の計画以上に進展している

理由

One of this year's big research goals was to find out the mechanism of how host insect Riptortus Pedestris and its Burkholderia gut symbiont maintain the successful symbiosis without harming each other. When I found the critical enzyme to maintain symbiosis named "Duox", I did not expect this enzyme is deeply related to stabilizing insect respiratory network. Since the discovery of novel function is difficult and valuable to understand the molecular mechanism of symbiosis in my field, I think I did a good job than my original expectation.

今後の研究の推進方策

During the last year, I screened many genes by RNAi technique to find vital symbiosis factor from both host- and symbiont-side. At that time, I found several interesting genes related to the development of the symbiotic organ. Some of them are known as important genes in other insects for another function (not important to the development of gut development), so my finding will be very novel that can regulate the symbiotic association. I will focus on research about these genes to find a new function which will be important to maintain the symbiotic association

  • 研究成果

    (9件)

すべて 2020 2019 その他

すべて 国際共同研究 (2件) 雑誌論文 (3件) (うち国際共著 3件、 査読あり 3件) 学会発表 (4件) (うち国際学会 2件)

  • [国際共同研究] Gachon University(韓国)

    • 国名
      韓国
    • 外国機関名
      Gachon University
  • [国際共同研究] CNRS(フランス)

    • 国名
      フランス
    • 外国機関名
      CNRS
  • [雑誌論文] Burkholderia insecticola triggers midgut closure in the bean bug Riptortus pedestris to prevent secondary bacterial infections of midgut crypts2020

    • 著者名/発表者名
      Kikuchi Yoshitomo、Ohbayashi Tsubasa、Jang Seonghan、Mergaert Peter
    • 雑誌名

      The ISME Journal

      巻: 14 ページ: 1627~1638

    • DOI

      10.1038/s41396-020-0633-3

    • 査読あり / 国際共著
  • [雑誌論文] Complete Genome Sequence of Burkholderia sp. Strain THE68, a Bacterial Symbiont Isolated from Midgut Crypts of the Seed Bug Togo hemipterus2020

    • 著者名/発表者名
      Takeshita Kazutaka、Jang Seonghan、Kikuchi Yoshitomo
    • 雑誌名

      Microbiology Resource Announcements

      巻: 9 ページ: -

    • DOI

      doi: 10.1128/MRA.00041-20

    • 査読あり / 国際共著
  • [雑誌論文] Host?symbiont specificity determined by microbe?microbe competition in an insect gut2019

    • 著者名/発表者名
      Itoh Hideomi、Jang Seonghan、Takeshita Kazutaka、Ohbayashi Tsubasa、Ohnishi Naomi、Meng Xian-Ying、Mitani Yasuo、Kikuchi Yoshitomo
    • 雑誌名

      Proceedings of the National Academy of Sciences

      巻: 116 ページ: 22673~22682

    • DOI

      doi: 10.1073/pnas.1912397116

    • 査読あり / 国際共著
  • [学会発表] Duox determines insect gut symbiosis by stabilizing respiratory network2019

    • 著者名/発表者名
      Jang Seonghan and Kikuchi Yoshitomo
    • 学会等名
      日本進化学会第21回北海道大会
  • [学会発表] A novel function of Duox in insect-microbe gut symbiosis: development of trachea network and stabilization of symbiotic association2019

    • 著者名/発表者名
      Jang Seonghan and Kikuchi Yoshitomo
    • 学会等名
      第63回日本応用動物昆虫学会大会
  • [学会発表] Gut commensal bacteria contribute insect’s healthcare by immune enhancement2019

    • 著者名/発表者名
      Jang Seonghan and Kikuchi Yoshitomo
    • 学会等名
      2019 Fall International Conference of KSAE
    • 国際学会
  • [学会発表] Host-symbiont specificity determined by microbe-microbe competition in an insect gut2019

    • 著者名/発表者名
      Jang Seongha, Itoh Hideomi, and Kikuchi Yoshitomo
    • 学会等名
      2019 Fall International Conference of KSAE
    • 国際学会

URL: 

公開日: 2021-01-27  

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