| 研究実績の概要 |
Arthritic synovial fibroblasts were isolated from the arthritic joints, defined by the lack of CD45, CD31, CD146 expression, but surface protein podoplanin (gp38) expression. We used these surface protein antibodies to distinguish fibroblasts from the other cell populations in arthritic joints. Within the fibroblasts populations, we used a novel RANKL reporter mice to purified the osteoclastogenic fibroblasts which express RANKL. We next performed RNA-seq and epigenetic analysis to characterize the RANKL- expressing fibroblasts in both genomic and epigenetic levels.
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