| 研究課題/領域番号 |
19J22546
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| 研究機関 | 東京大学 |
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研究代表者 |
寿 景文 東京大学, 工学系研究科, 特別研究員(DC1)
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| 研究期間 (年度) |
2019-04-25 – 2022-03-31
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| キーワード | super-multiplex / stimulated Raman / fluorescence / high-speed |
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| 研究実績の概要 |
We have worked on the theme of ‘super-multiplex biological imaging’ towards different directions of development.
1. We report integrated stimulated Raman and fluorescence microscopy with simultaneous multimodal color tunability at high speed, enabling super-multiplex imaging covering diverse molecular contrasts with temporal resolution of seconds.
2. We demonstrate photoswitchable stimulated Raman scattering spectroscopy and microscopy where narrowband vibrational signatures are switched with full reversibility at high speed.
3. We developed four types of activatable Raman probes, which are targeted to different enzymes and tuned to different vibrational frequencies by isotope editing of the nitrile group.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
To achieve the initial topic of 'High-speed super-multiplex organelle imaging for interactome analysis', we have developed the integrated stimulated Raman and fluorescence microscopy with fast programmable tunability.
By exploiting the advanced imaging system, we are able to observe up to eight kinds of subceullar organelles simultaneously with temporal resolution of seconds.
Besides, we also built a novel techniques called photoswitchable stimulated Raman scattering which is desired for super-multiplex super-resolution imaging. Also, we developed new kinds of Raman probes for enzyme imaging.
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| 今後の研究の推進方策 |
In the last year, we are going to work on the publication of the achievements. Despite the journal papers we have submitted, we plan to attend more conference to share our results and build helpful connnection with other researches in different research fields.
Furthermore, we will develop novel light sources with faster and wider tunability to enhance the high-speed super-multiplex imaging more. Also, we will modify the photoswitch stimulated Raman scattering technologies towards super-multiplex super-resolution imaging which is highly promising to bring new bioligical insights.
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