| 研究課題/領域番号 |
19K06620
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
MARLETAZ Ferdinand 沖縄科学技術大学院大学, 分子遺伝学ユニット, 客員研究員 (90833955)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | development / Hox / evolution / cephalopod / chromatin / gene expression |
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| 研究実績の概要 |
We have continue the analysis of the E. berryi genome and the annotation of developmental genes by performing gene family reconstruction, domain annotation and phylogenetic analysis for a number of developmental genes.
We started the sequencing of the samples and realised that we had to optimise the protocol a bit. In the meantime, we decided to try to develop a spatial transcriptomics approach which could be insightful to detect differentially expressed genes during squid limb development. We also analysed HiC chromatin conformation data around Hox cluster of the squid. We also started development HCR in situ hybridisation to optimise gene expression characterisation in squid.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The covid has complicated the procurement of biological samples this year.
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| 今後の研究の推進方策 |
We will sequence more samples next year and start gene expression monitoring of candidates.
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| 次年度使用額が生じた理由 |
Sequencing remaining samples and resupply of reagents
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