研究実績の概要 |
Our plan was to obtain RNA-seq and ATAC-seq library for each limb bud of a bobtail squid embryo in order to predict differences in transcription factor binding that would be involved in arm identity. We wanted to understand how a novel patterning could be deployed . We performed these experiments and sequenced corresponding libraries. We performed peak calling and differential peak activation analysis, which has been coupled with foot printing analysis. We identified some key genes and ordered corresponding probes for HCR, an advanced in situ hybridisation technique. In parellel, we optimised protocols for HCR imagining of squid embryos and fixed a lot of embryos. Some key results have been integrated in a paper about squid single-cell sequencing that will be submit in 2022.
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