| 研究課題/領域番号 |
19K06701
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| 研究機関 | 北海道大学 |
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研究代表者 |
Teh Ooikock 北海道大学, 高等教育推進機構, 助教 (80791277)
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| 研究分担者 |
藤田 知道 北海道大学, 理学研究院, 教授 (50322631)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | planar cell polarity / Physcomitrella patens / glycosylation |
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| 研究実績の概要 |
In the past one year, we have successfully achieved the following objectives: (1) We completed the Prince Charming (PC) genetic screen and isolated 36 PC mutants. We are currently focusing on 2 PC mutants, namely PC8 and PC36. We are in the process of generating a mapping population for whole genome sequencing. (2) We have generated a series of glycosylation mutant for Sleeping BEauty (SB) through point mutagenesis. We found that mutation of 5 putative glycosylation sites totally abolish the SB function in promoting polarized tip growth. (3) We have identified ARFC2 as a downstream target for the SB-dependent signaling. ARFC2 knock-out mutant generated also show phenotype which is consistent with the SB overexpressor.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
So far we have been able to stick to the original proposed plan.
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| 今後の研究の推進方策 |
In the next fiscal year, we plan to identify the causative mutations of the two selected PC mutants by engaging commercial sequencing services. We also plan to complete the SB interactome.
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| 次年度使用額が生じた理由 |
Due to some unforeseen circumstances, we are able to make progress in the research plan by spending less budget. However this year we envisage to spend the money fully to achieve even greater progress.
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