| 研究実績の概要 |
Characterization of a pyridoxamine (PM)-dopamine (DA) adduct:
1. In the reaction of DA and PM, the (pyridoxal) PL-DA adduct was formed together with PL. The adduct corresponded to PL+DA-H2O was also produced in the reaction of DA and PL. Its structure was characterized to be a 1-[3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl-1,2,3,4-tetrahydroisoquinoline-6,7-diol formed through a Schiff base by MS/MS, UV, and NMR analyses.
2. Based on the results from the reaction of DA analogs and PM, the mechanism for the formation of PL-DA adduct was proposed. Thus, DA initially oxidizes to DA quinone (DAQ), which reacts with PM to produce PL after hydrolysis. PL then reacts with DA to form PL-DA adduct.
3. As the PM concentration increased, dose-dependent elevation of a PL-DA adduct was observed.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
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理由
As planned, DA was allowed to react with PM and the adduct (PL+DA-H2O) was characterized by MS/MS, UV, and NMR analyses. The mechanism for the formation of adduct was also proposed based on the reaction of DA analogs and PM. Dose-dependent elevation of a PL-DA adduct was observed. Therefore, PM could inhibit DA-induced neurotoxicity by directly scavenging DAQ.
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