| 研究課題/領域番号 |
19K07586
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| 研究機関 | 国立感染症研究所 |
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研究代表者 |
アリ フセインハッサン 国立感染症研究所, ウイルス第二部, 主任研究官 (00523515)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | HBx / Ski2 / RNA degradation / HBV / Viral life cycle |
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| 研究実績の概要 |
By controlling mRNA stability, the virus can manipulate cellular environment to favor its persistence.Ski2 is an essential helicase for cytoplasmic RNA degradation through the RNA exosome system. HBx regulated Ski2 expression.These data suggested a possible effect of HBx on the mRNA stability,and the resulting regulation of gene expression, for both viral and host RNA that is degraded through the RNA exosome system.In 2019, we analyzed the role of HBx on the regulation of other HBV proteins expression through the regulation of Ski2-mediated mRNA decay (using expressing vectors for HBV, HBV lacking the expression of HBx, and siRNA targeting Ski2). Functional analysis is undergoing to analyze its regulation on the viral life cycle.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We clarified the role of HBx on the expression of other HBV proteins through the regulation of Ski2-mediated RNA degradation in 2019. All tools required to perform the study were available, and the financial support was enough to reach this target.
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| 今後の研究の推進方策 |
In 2020, we aim to perform two major lines of experiments. On the viral side, we wish to clarify the functional significance of HBx-mediated regulation of the expression of other HBV proteins through its regulation of RNA-Exosome mediated RNA degradation. While on the host side, we will perform deep sequencing to identify the host RNA transcriptome that is affected by HBx regulation of RNA-exosome mediated RNA degradation; confirm RNA-sequencing results by quantitative real time PCR, and start to analyze possible role of these host genes on viral life cycle, and/or viral-induced carcinogenesis.
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