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2022 年度 実施状況報告書

Exploring the novel role of mitochondrial dynamics in schizophrenia

研究課題

研究課題/領域番号 19K08041
研究機関和歌山県立医科大学

研究代表者

岩田 圭子  和歌山県立医科大学, 薬学部, 講師 (30415088)

研究分担者 松崎 秀夫  福井大学, 子どものこころの発達研究センター, 教授 (00334970)
研究期間 (年度) 2019-04-01 – 2024-03-31
キーワードoligodendrocyte / mitochondria / schizophrenia
研究実績の概要

In this project, we wish to address the impact of changes in mitochondrial biogenesis on central nervous system (CNS) development (especially oligodendrocyte differentiation) and animal behavior. Neurons and synapses have long been the dominant focus of neuroscience, informing theories on the pathophysiology of psychiatric disorders. However, the majority of cells in the brain are not neurons but glial cells. We are performing experiments to address how oligodendrocyte differentiation regulated by mitochondria impacts the higher brain functions and pathophysiology of schizophrenia (SZ) following our research methodology and approach.
Here, we report new findings by the 4th year of the project as follows;
We identified the novel variant of PGC-1alpha, a transcriptional coactivator, which is a key regulator of mitochondrial biogenesis and was involved in oligodendrocyte differentiation using the human oligodendrocyte cell line. The variant was expressed in the human brain. We performed RNA sequences using human brain samples. (The study was Fukui University Medical Research Ethics Committee (approval no.2020028).) The expression of the variant was correlated with 2 transcriptional receptors which were involved in cell differentiation. Now we are conducting in vivo and in vitro experiments using mice. We established the primary culture system of oligodendrocytes at Wakayama Medical Univ and confirmed that the expression of the novel variant was increased during the differentiation of the mouse oligodendrocyte precursor cells.

現在までの達成度 (区分)
現在までの達成度 (区分)

3: やや遅れている

理由

As we planned, we established the primary culture system of oligodendrocytes at Wakayama Medical Univ and confirmed that the expression of the novel variant was increased during the differentiation of the mouse oligodendrocyte precursor cells. On the other hand, we could not visit the Lab of CR (Prof. Scorrano), Padova University, Italy, and conduct experiments, especially mitochondrial analyses using imaging systems that are available in his Lab, these experiments are a little behind schedule.

今後の研究の推進方策

I will visit the Lab of CR (Prof. Scorrano), Padova University, Italy, and conduct experiments following the original plan.

次年度使用額が生じた理由

As it is written in Current Status, it is needed to conduct experiments, especially mitochondrial analyses using imaging systems that are available in the Lab of CR (Prof. Scorrano), Padova University, Italy. I will visit the Lab and conduct experiments following the original plan.

  • 研究成果

    (5件)

すべて 2023 2022 その他

すべて 国際共同研究 (1件) 雑誌論文 (1件) (うち国際共著 1件、 査読あり 1件) 学会発表 (2件) 備考 (1件)

  • [国際共同研究] Veneto Institute of Molecular Medicine/University of Padova(イタリア)

    • 国名
      イタリア
    • 外国機関名
      Veneto Institute of Molecular Medicine/University of Padova
  • [雑誌論文] Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)2022

    • 著者名/発表者名
      Iwata Keiko、Xie Min-Jue、Guest Paul C.、Hirai Takaharu、Matsuzazki Hideo
    • 雑誌名

      Methods Mol Biol.

      巻: 2511 ページ: 321~332

    • DOI

      10.1007/978-1-0716-2395-4_24

    • 査読あり / 国際共著
  • [学会発表] 神経系の細胞分化における ミトコンドリアダイナミクスの役割2023

    • 著者名/発表者名
      岩田圭子, 松﨑秀夫, 新谷紀人
    • 学会等名
      第11回ニューロカンファレンス和歌山
  • [学会発表] Exploring the novel role of mitochondria dynamics in neuron and oligodendrocyte differentiation.2022

    • 著者名/発表者名
      Keiko Iwata,Hideo Matsuzaki,Masahumi Moguchi,Norihito Shintani
    • 学会等名
      第96回日本薬理学会年会
  • [備考] researchmap

    • URL

      https://researchmap.jp/keikoiwata

URL: 

公開日: 2023-12-25  

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