| 研究課題/領域番号 |
19K08954
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| 研究機関 | 長崎大学 |
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研究代表者 |
モイ メンリン 長崎大学, 熱帯医学研究所, 教授 (40597499)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | デング熱 / miRNA |
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| 研究実績の概要 |
The proposed research strategy involves the following three components (clinical, virologic and immunologic). We have collected 100 serum samples from patients with dengue and severe dengue in Vietnam. Ascites fluid was collected from 40 patients with severe dengue. Inflammatory cytokines were determined by a panel of 10 cytokines to determine inflammatory mediators according to disease severity. Using 8 serum samples and 8 ascites samples, miRNA was isolated and miRNA expression was determined by using microarray. Selected samples will also be analysed by RNAseq, followed by validation by qPCR.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Dengue patient samples for the characterization of miRNA was been collected together with clinical data. Currently, samples were processed for further analyses by miRNA microarray and RNAseq, as planned in the proposal.
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| 今後の研究の推進方策 |
In the following experiments, levels of antibodies will be determined by using ELISA and neutralizing assay. Because disease outcomes are also affected by sequence of infection, these data will be used to group patients according to disease severity, course of disease and type of infection and analyzed with levels of expressed miRNA. Next, miRNA expression will be determined by RNAseq and microarray by using samples from blood and ascites.
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