| 研究実績の概要 |
The proposed research strategy involves the following three components (clinical, virologic and immunologic). We have collected 100 serum
samples from patients with dengue and severe dengue in Vietnam, Myanmar and Japan. Using a panel of 10 cytokines, inflammatory mediators were first tested to determine cytokine levels and classify it according to disease severity. Using PCA studies, we were able to group sampmles according to severity, particularly for samples that were negative for virus RNA. miRNA was then isolated from 6 acute and 6 convalescent samples, and a microarray was used to examine the miRNAs and ranking used to determine miRNAs that were overly expressed during the acute disease phase.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Due to COVID-19 restrictions, our collaborators were not able to further collect patient samples according to schedule. While the sample collection phase of the project has been delayed for another year, the miRNA analyses will continue with samples collected prior to the implementation of the COVID-19 movement restrictions.
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