| 研究課題/領域番号 |
19K10044
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| 研究機関 | 愛媛大学 |
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研究代表者 |
李 智媛 愛媛大学, プロテオサイエンスセンター, 助教(特定教員) (70711274)
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| 研究期間 (年度) |
2019-04-01 – 2023-03-31
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| キーワード | SWI/SNF / BAF155 / Histomorphometry / Rank / Lysosome |
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| 研究実績の概要 |
Generation of cKO mice in FY2019; SWI/SNF (chromatin remodeler) complex is essential gene and conventional deletion leads to embryonic lethality. As planed in proposal, we generated LysM-Cre;Baf155f/f and Rank-Are; Baf155f/f mice which are specific deletion for osteoclast progenitor and precursor, respectively. We studied bone remodeling in adult mice of 8weeks female and male. We examined the bone mineral density by microCT and bone histomorphometry using femoral bone sections. Each parameters for osteoclastic and bone resorption were intensively score.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our analysis is progressing as planed in proposal. Currently we design for RNA-Seq analysis and will perform the data mining.
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| 今後の研究の推進方策 |
From analysis of RNA-seq (or ChIP-seq if we need), we will analysis for data mining.
We also examine the osteoclast ontogeny. For in vitro assays, we harvest bone marrow cells from mouse tibia, and then culture them with M-CSF and RANKL and allow to proliferate and differentiation into osteoclast activation. We will perform cell morphology observation during cell activation, which are affect from chromatin remodeling.
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| 次年度使用額が生じた理由 |
Transcriptomic analysis like RNA-Seq and ChIP-Seq will perform in FY2020.
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