| 研究実績の概要 |
In this research, we investigated the functional role of epigenetic chromatin remodeler, BAF155 in osteoclasts and bone metabolism. By analysis of microCT and bone histomorphometry of 16-week-old male mice, we confirmed that the Baf155 deficient mice significantly increased the TRAP5b and CTX which are the representative serum markers of bone resorption, and thereby decreased the bone mineral density. By using human osteoclasts, we demonstrated that BAF155 expression during osteoclast differentiation stages. The BAF155 expression was significantly decreased in mature osteoclast, not bone marrow macrophage or osteoclast precursor stage, in contrast BAF170 expression was increased. Also, we found several target candidates from RNA sequencing analysis. We are investigating the validation of candidates by RT-PCR and immunoblotting.
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