| 研究課題/領域番号 |
19K10687
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| 研究機関 | 香川大学 |
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研究代表者 |
モストファ ジャーマル 香川大学, 医学部, 助教 (50418802)
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| 研究分担者 |
塚本 郁子 香川大学, 医学部, 寄付講座教員 (10183477)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | MPTP / Mortality / Monoamines / MPP / cell culture / cell viability |
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| 研究実績の概要 |
1. Optimization of MPTP dose in mice:
The mortality at a 20 mg/kg dose was substantial, from 90-100%. At 15 mg/kg, the mortality rate is reduced to 50%. The result showed that dopamine, DOPAC and HVA were reduced to 60-70% in the striatum using HPLC-ECD.
2. Cell viability assay in PC12 cells:
Incubation with various concentrations of MPP+ (0, 200, 500, and 1000 uM) at 24 and 48 h did not significantly affect viable cell count and the LDH activity, indicating that MPP+ does not cause PC12 cell toxicity.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
MPTP is a highly neurotoxic compound. MPTP preparation and its administration to animal should be conducted in a fume hood. From April 2020-March 2021, the draft chamber is not available due to the construction of the animal building.
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| 今後の研究の推進方策 |
April 2020-March 2021: a. HPLC-ECD analysis for dopamine release in MPP treated PC12 cells, b. Immunoblot for TH phosphorylation at Ser31 and Ser40, PARP-1 and caspase in MPP treated PC12 cells.
April 2021-March 2022: a. HPLC-ECD analysis of dopamine, serotonin and their metabolites in MPTP treated mice, b.Immunoblot for TH phosphorylation at Ser31 and Ser40, PARP-1 and caspase in MPTP treated mice
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| 次年度使用額が生じた理由 |
To peruse cell culture, need to buy the following things
1. PC12 cells, 2. Antibodies for WB, 3. IBend Flex western kit
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