| 研究課題/領域番号 |
19K12801
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| 研究機関 | 大阪大学 |
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研究代表者 |
李 俊君 大阪大学, 医学系研究科, 特任助教(常勤) (10723786)
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| 研究分担者 |
南 一成 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537) [辞退]
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | pluripotent stem cells / cardiomyocytes / maturation |
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| 研究実績の概要 |
As for this year, we planned to prepare the matured self-organized tissue rings and evaluate their therapeutic effect on the rat heart with myocardium infarction. Although the project has been affected by the COVID-19, we managed to get the following the achievements:
1.Improved from the matured tissue ring, the 3D tissue sheet paced by the traveling wave have been prepared. The traveling wave could be maintained for over two weeks. This new process allows improved tissue organization and easier transplantation.
2.We proved that the tissue paced by the traveling wave could have better survival rate in hypoxia than the control group. And this could be confirmed by the upregulated expression of metabolism related gene.
3.One paper has been published with the support of grant.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The project has been affected by the pandemic of COVID-19. The lab has been temporally out of service, and some lab members related to this project have to work remotely in home during the most serious phase of pandemic. The order of animals needed for the transplantation were delayed as well.
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| 今後の研究の推進方策 |
Based on the previous work, we will carry out the following procedures:
1.To transplant the 3D tissue matured by traveling wave to the rat heart with myocardial infarction. 2. To optimize the transplantation condition, such as cell number, tissue size and transplantation timing. 3. To monitor the heart function for over one month post transplantation and to evaluate the retention of transplanted cardiac tissue. 4. To investigate the correlation between the maturation level (such as gene expression, protein expression, contraction motion, electrophysiology and so on) and the retention of the transplanted cells as well as the functional recovery of the infarcted heart.
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| 次年度使用額が生じた理由 |
COVID-19のため、計画されているすべての助成金を使用することはできません。 今年の残りの助成金は来年に使用されます。
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