自発進行波による高成熟心筋を用いた新規心筋梗塞治療法の開発

2021 年度 実績報告書

• 研究課題/領域番号: 19K12801
• 研究機関: 大阪大学
• 研究代表者: 李 俊君 大阪大学, 医学系研究科, 特任助教(非常勤) (10723786)
• 研究分担者: 南 一成 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537) [辞退]
• 研究期間 (年度): 2019-04-01 – 2022-03-31
• キーワード: pluripotent stem cells / cardiomyocytes / maturation / regenerative medicine

研究実績の概要

We planned to prepare the matured self-organized tissue and transplant them into a rat heart with myocardial infarction. We planned to understand the mechanism underlying the therapeutic effect of matured cardiac tissue. The achievements: (1)The mature tissue group demonstrated an improved therapeutic effect on the rat heart with myocardial infarction compared with the control group. (2) Compared with the control group, the matured cardiac tissue demonstrated an enhanced survival after hypoxia culture. In addition, we find the improved survival could be related to the higher expression of myoglobin, cytochrome c, important proteins for cells to receive/use the oxygen. (3) We published/applied 1 paper and 1 patent and gave an oral presentation in 2021 American Heart Association conference.

研究成果

• [雑誌論文] Therapeutic efficacy of large aligned cardiac tissue derived from induced pluripotent stem cell in a porcine ischemic cardiomyopathy model (2021)
  • 著者名/発表者名: Suzuki Kota、Miyagawa Shigeru、Liu Li、Kawamura Takuji、Li Junjun、Qu Xiang、Harada Akima、Toda Koichi、Yoshioka Daisuke、Kainuma Satoshi、Kawamura Ai、Sawa Yoshiki
  • 雑誌名: The Journal of Heart and Lung Transplantation 巻: 40 ページ: 767～777
  • DOI: 10.1016/j.healun.2021.04.010
  • 査読あり
• [学会発表] Human iPSC derived cardiac tissue matured by spontaneous traveling waves for improved drug assessment (2021)
  • 著者名/発表者名: 1.Li, J., Liu, L., Miyagawa, S., Kawamura, T., Matsuura, R., Hua, Y., Qu, X., Sougawa, N., Sawa, Y.,
  • 学会等名: American Heart Association Scientific Session, (2021)
  • 国際学会
• [産業財産権] 心筋細胞層の製造方法、心筋細胞層、およびその利用 (2021)
  • 発明者名: 劉莉、澤芳樹、宮川繁、李俊君、武田真季、三好隼人
  • 権利者名: 劉莉、澤芳樹、宮川繁、李俊君、武田真季、三好隼人
  • 産業財産権種類: 特許
  • 産業財産権番号: 2021-176040